Data di Pubblicazione:
1985
Citazione:
Reconstitution of proton pumping activity of a plasma membrane ATPase purified from radish / M.C Cocucci, M.I. De Michelis, M.C. Pugliarello, F. Rasi-Caldogno. - In: PLANT SCIENCE LETTERS. - ISSN 0304-4211. - 37:3(1985), pp. 189-193.
Abstract:
Plasma membrane ATPase partially purified from radish seedlings (Raphanus sativum L.) (2.4–3.5 μmol Pi min−1 mg−1 protein) has been reconstituted in proteoliposomes by the cholate-dialysis technique. Proteoliposomes are able to acidify their internal volume in the presence of Mg:ATP. Mg:ATP-dependent proton pumping is prevented by N,N′-dicyclohexylcarbodiimide (DCCD) and by vanadate at the same concentrations which are effective on the phosphohydrolyzing activity of the plasma membrane ATPase.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
H+-ATPase; plasma membrane ATPase; proteoliposomes; proton transport; radish; vanadate
Elenco autori:
M.C Cocucci, M.I. De Michelis, M.C. Pugliarello, F. Rasi-Caldogno
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