Gene transfer into human dendritic antigen-presenting cells by vaccinia virus and adenovirus vectors
Articolo
Data di Pubblicazione:
1998
Citazione:
Gene transfer into human dendritic antigen-presenting cells by vaccinia virus and adenovirus vectors / M. Di Nicola, S. Siena, M. Bregni, P. Longoni, M. Magni, M. Milanesi, P. Matteucci, R. Mortarini, A. Anichini, G. Parmiani, I. Drexler, V. Erfle, G. Sutter, A. M. Gianni. - In: CANCER GENE THERAPY. - ISSN 0929-1903. - 5:6(1998), pp. 350-6-356.
Abstract:
In a search for means to deliver exogenous gene(s) into human dendritic cells (DCs) from the perspective of tumor-specific vaccination, we have evaluated two recombinant viruses, both of which carry a reporter gene which is namely a modified vaccinia virus Ankara (MVA) and an adenovirus, as possible expression vectors. The recombinant MVA-P11 LZ vector carries the Escherichia coli lacZ gene coding for the enzyme beta-galactosidase, and the recombinant Ad-MFG-AP vector carries a modified membrane-exposed alkaline phosphatase (AP) gene. DCs were generated ex vivo in the presence of tumor necrosis factor-alpha, granulocyte macrophage colony-stimulating factor, stem cell factor, and flk-2/flt-3 ligand taken from CD34+ hematopoietic progenitors that were mobilized into the peripheral blood of cancer patients treated with high-dose cyclophosphamide and filgrastim. The target cells used for gene delivery were either CD34+ cells that had been subsequently induced to differentiate into mature DCs or DCs transduced after ex vivo generation from CD34+ cells. The results showed that: (a) infection of CD34+ cell derived-DCs (mature DCs) with either viral vector resulted in the efficient synthesis of recombinant protein, and (b) CD34+ cells were permissive for the expression of the recombinant reporter gene after infection with Ad-MFG-AP but not after infection with MVA-P11 LZ. In conclusion, these results suggest that vaccinia and adenovirus vectors are candidate to act as vehicles in genetically engineering human DCs.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Adenoviridae; Dendritic Cells; Gene Transfer Techniques; Dose-Response Relationship, Drug; Humans; Transduction, Genetic; Antigens, CD34; Vaccinia virus; Genetic Vectors; Flow Cytometry; Antigen-Presenting Cells; Stem Cells; Fluorescent Antibody Technique; Time Factors; Female
Elenco autori:
M. Di Nicola, S. Siena, M. Bregni, P. Longoni, M. Magni, M. Milanesi, P. Matteucci, R. Mortarini, A. Anichini, G. Parmiani, I. Drexler, V. Erfle, G. Sutter, A. M. Gianni
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