Potato virus X movement in Nicotiana benthamiana: new details revealed by chimeric coat protein variants
Articolo
Data di Pubblicazione:
2012
Citazione:
Potato virus X movement in Nicotiana benthamiana: new details revealed by chimeric coat protein variants / C. Betti, C. Lico, D. Maffi, S. D'Angeli, M. M. Altamura, E .Benvenuto, F. Faoro, S. Baschieri. - In: MOLECULAR PLANT PATHOLOGY. - ISSN 1464-6722. - 13:2(2012), pp. 198-203. [10.1111/J.1364-3703.2011.00739.X]
Abstract:
Potato virus X coat protein is necessary for both cell-to-cell and phloem transfer, but it has not been clarified definitively whether it is needed in both movement phases solely as a component of the assembled particles or also of differently structured ribonucleoprotein complexes. To clarify this issue, we studied the infection progression of a mutant carrying an N-terminal deletion of the coat protein, which was used to construct chimeric virus particles displaying peptides selectively affecting phloem transfer or cell-to-cell movement. Nicotiana benthamiana plants inoculated
with expression vectors encoding the wild-type, mutant and chimeric viral genomes were examined by microscopy techniques.
These experiments showed that coat protein–peptide fusions promoting
cell-to-cell transfer only were not competent for virion assembly, whereas long-distance movement was possible only for coat proteins compatible with virus particle formation. Moreover, the ability of the assembled PVX to enter and persist into developing xylem elements was revealed here for the first time
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
plant vaccines ; virus movement ; Nicotiana benthamiana ; PVX
Elenco autori:
C. Betti, C. Lico, D. Maffi, S. D'Angeli, M.M. Altamura, E. Benvenuto, F. Faoro, S. Baschieri
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