Data di Pubblicazione:
2012
Citazione:
Widespread generation of alternative UTRs contributes to sex-specific RNA binding by UNR / M. Mihailovich, L. Wurth, F. Zambelli, I. Abaza, C. Militti, F.M. Mancuso, G. Roma, G. Pavesi, F. Gebauer. - In: RNA. - ISSN 1355-8382. - 18:1(2012 Jan), pp. 53-64.
Abstract:
Upstream of N-ras (UNR) is a conserved RNA-binding protein that regulates mRNA translation and stability by binding to sites generally located in untranslated regions (UTRs). In Drosophila, sex-specific binding of UNR to msl2 mRNA and the noncoding RNA roX is believed to play key roles in the control of X-chromosome dosage compensation in both sexes. To investigate broader sex-specific functions of UNR, we have identified its RNA targets in adult male and female flies by high-throughput RNA binding and transcriptome analysis. Here we show that UNR binds to a large set of protein-coding transcripts and to a smaller set of noncoding RNAs in a sex-specific fashion. The analyses also reveal a strong correlation between sex-specific binding of UNR and sex-specific differential expression of UTRs in target genes. Validation experiments indicate that UNR indeed recognizes sex-specifically processed transcripts. These results suggest that UNR exploits the transcript diversity generated by alternative processing and alternative promoter usage to bind and regulate target genes in a sex-specific manner.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
UNR; X-chromosome; dosage compensation; RIP-Seq
Elenco autori:
M. Mihailovich, L. Wurth, F. Zambelli, I. Abaza, C. Militti, F.M. Mancuso, G. Roma, G. Pavesi, F. Gebauer
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