Association of lung adenocarcinoma clinical stage with gene expression pattern in noninvolved lung tissue
Articolo
Data di Pubblicazione:
2012
Citazione:
Association of lung adenocarcinoma clinical stage with gene expression pattern in noninvolved lung tissue / E. Frullanti, F. Colombo, F.S. Falvella, A. Galvan, S. Noci, L. De Cecco, M. Incarbone, M. Alloisio, L. Santambrogio, M. Nosotti, D. Tosi, U. Pastorino, T.A. Dragani. - In: INTERNATIONAL JOURNAL OF CANCER. - ISSN 0020-7136. - 131:5(2012 Sep), pp. E643-E648.
Abstract:
Associations between clinical outcome of cancer patients and the gene expression signature in primary tumors at time of
diagnosis have been reported. To test whether gene expression patterns in noninvolved lung tissue might correlate with
clinical stage in lung adenocarcinoma (ADCA) patients, we compared the transcriptome of noninvolved lung samples from
60 ADCA smoker patients of clinical stage I versus 60 patients with stage >I. Quantitative PCR of 10 genes with the most
significant differential expression confirmed the statistical association with clinical stage in eight genes, six of which were
downregulated in high-stage patients. Five of these six genes were also downregulated in lung ADCA tissue as compared to
noninvolved tissue. Studies in vitro indicated that four of the genes (SLC14A1, SMAD6, TMEM100 and TXNIP) inhibited colony
formation of lung cancer cell lines transfected to overexpress the genes, suggesting their potential tumor-suppressor activity.
Our findings suggest that individual variations in the transcriptional profile of noninvolved lung tissue may reflect the lung
ADCA patient’s predisposition to tumor aggressiveness.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
lung adenocarcinoma; gene expression profile; transcriptome; tumor progression
Elenco autori:
E. Frullanti, F. Colombo, F.S. Falvella, A. Galvan, S. Noci, L. De Cecco, M. Incarbone, M. Alloisio, L. Santambrogio, M. Nosotti, D. Tosi, U. Pastorino, T.A. Dragani
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