Data di Pubblicazione:
2012
Citazione:
Prednisolone and prednisone neo-formation in bovine urine after sampling / F. Arioli, A. Casati, M. Fidani, M. Silvestri, G. Pompa. - In: ANIMAL. - ISSN 1751-7311. - 6:6(2012 Jun), pp. 1023-1029. [10.1017/S1751731111002497]
Abstract:
The rise in the frequency of detecting prednisolone in bovine urine from northern Italy has come into focus of attention in recent
years. The possibility that neo-formation of prednisolone or that prednisone may occur in urine after collection of samples was
therefore investigated. Cow urine collected for official routine controls in Lombardy containing more than 80 ng/ml cortisol,
and prednisolone and prednisone below the decision limit (CCa) of the method (0.4 and 0.5 ng/ml, respectively) was used.
The C1–2 dehydrogenation of naturally present cortisol and cortisone was checked by incubating urine, both contaminated and
uncontaminated with faeces, at 378C and by collecting samples at 0, 1, 2, 4, 6 and 24 h. The influence of Helix pomatia juice
was also investigated in order to determine whether deconjugation could influence the reliability of the results. All samples were
analysed by HPLC-MS3 for the presence of cortisol, cortisone, prednisolone and prednisone in negative electrospray ionisation
mode, utilising the consecutive reaction monitoring of product ions derived from the formate molecular adduct ([M1HCOO]–).
The observed neo-formation of prednisolone shows that inappropriate temperatures in sample storage and processing can result
in an incorrect accusation of non-compliance. The faecal contamination of urine, performed with the aim to mimic a collection
conducted without the necessary care, moreover, evoked a high increase in prednisolone concentration in two out of seven
animals. Moreover, H. pomatia juice had no significant effect on the prednisolone concentration, indicating that this
corticosteroid is present in its free form in cow urine.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
bovine urine ; prednisolone ; prednisone ; cortisol ; cortisone
Elenco autori:
F. Arioli, A. Casati, M. Fidani, M. Silvestri, G. Pompa
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