Molecular characterisation of Cryptosporidium isolates from pre-weaned calves in Romania : is there an actual risk of zoonotic infections?
Articolo
Data di Pubblicazione:
2011
Citazione:
Molecular characterisation of Cryptosporidium isolates from pre-weaned calves in Romania : is there an actual risk of zoonotic infections? / K. Imre, L.M. Lobo, O. Matos, C. Popescu, C. Genchi, G. Dărăbuş. - In: VETERINARY PARASITOLOGY. - ISSN 0304-4017. - 181:2-4(2011 Sep 27), pp. 321-324. [10.1016/j.vetpar.2011.04.042]
Abstract:
Diarrheic fecal samples from 258 pre-weaned calves (1-30 day-old) from 9 dairy
farms located in Banat region, Romania, were microscopically examined for the
presence of Cryptosporidium oocysts. Overall, 65 (25%) samples were found
positive. A higher percent of infection was recorded in calves aged between 8 and
14 days compared with other age categories (1-7, 8-14, 15-21 and 22-30 days;
p<0.05). Genetic characterization was carried out on all Cryptosporidium-positive
samples. After DNA extraction, Cryptosporidium species were determined by a
nested PCR of the small subunit rRNA gene (18S) followed by RFLP analysis with
SspI, VspI and MboII restriction enzymes. The restriction patterns showed that
animals were infected with Cryptosporidium parvum. Subsequently, subtyping of 13
C. parvum isolates, based on sequence analysis of the 60 kDa glycoprotein (GP60)
gene, showed 2 subtypes (IIaA15G2R1 and IIaA16G1R1) belonging to the subtype
family IIa. This is the first molecular study of bovine Cryptosporidium infection
in Romania.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cryptosporidium ; pre-weaned calves ; SSU rRNA ; PCR-RFLP ; CP60 subtypes
Elenco autori:
K. Imre, L.M. Lobo, O. Matos, C. Popescu, C. Genchi, G. Dărăbuş
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