Data di Pubblicazione:
2007
Citazione:
Salicylic acid activates nitric oxide synthesis in Arabidopsis / M. Zottini, A. Costa, R. De Michele, M. Ruzzene, F. Carimi, F. Lo Schiavo. - In: JOURNAL OF EXPERIMENTAL BOTANY. - ISSN 0022-0957. - 58:6(2007 May), pp. 1397-1405.
Abstract:
The relationship between nitric oxide (NO) and salicylic
acid (SA) was investigated in Arabidopsis thaliana.
Here it is shown that SA is able to induce NO synthesis
in a dose-dependent manner in Arabidopsis. NO production
was detected by confocal microscopic analysis
and spectrofluorometric assay in plant roots
and cultured cells. To identify the metabolic pathways
involved in SA-induced NO synthesis, genetic and pharmacological
approaches were adopted. The analysis
of the nia1,nia2 mutant showed that nitrate reductase
activity was not required for SA-induced NO production.
Experiments performed in the presence of a nitric
oxide synthase (NOS) inhibitor suggested the involvement
of NOS-like enzyme activity in this metabolic
pathway. Moreover, the production of NO by SA treatment
of Atnos1 mutant plants was strongly reduced
compared with wild-type plants. Components of the SA
signalling pathway giving rise to NO production were
identified, and both calcium and casein kinase 2 (CK2)
were demonstrated to be involved. Taken together,
these results suggest that SA induces NO production
at least in part through the activity of a NOS-like enzyme
and that calcium and CK2 activity are essential
components of the signalling cascade.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Arabidopsis thaliana; Atnos1; Casein kinase 2; nia1,nia2; Nitrate reductase; Nitric oxide; Salicylic acid signals
Elenco autori:
M. Zottini, A. Costa, R. De Michele, M. Ruzzene, F. Carimi, F. Lo Schiavo
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