Nitric Oxide Is Involved in Cadmium-Induced Programmed Cell Death in Arabidopsis Suspension Cultures
Articolo
Data di Pubblicazione:
2009
Citazione:
Nitric Oxide Is Involved in Cadmium-Induced Programmed Cell Death in Arabidopsis Suspension Cultures / R. De Michele, E. Vurro, C. Rigo, A. Costa, L. Elviri, M. Di Valentin, M. Careri, M. Zottini, L.S. di Toppi, F. Lo Schiavo. - In: PLANT PHYSIOLOGY. - ISSN 0032-0889. - 150:1(2009 May), pp. 217-228.
Abstract:
Exposure to cadmium (Cd2+) can result in cell death, but the molecular mechanisms of Cd2+ cytotoxicity in plants are not fully
understood. Here, we show that Arabidopsis (Arabidopsis thaliana) cell suspension cultures underwent a process of programmed
cell death when exposed to 100 and 150 mM CdCl2 and that this process resembled an accelerated senescence, as suggested by the
expression of the marker senescence-associated gene12 (SAG12). CdCl2 treatment was accompanied by a rapid increase in nitric
oxide (NO) and phytochelatin synthesis, which continued to be high as long as cells remained viable. Hydrogen peroxide
production was a later event and preceded the rise of cell death by about 24 h. Inhibition of NO synthesis by NG-monomethylarginine
monoacetate resulted in partial prevention of hydrogen peroxide increase, SAG12 expression, and mortality, indicating
that NO is actually required for Cd2+-induced cell death. NO also modulated the extent of phytochelatin content, and possibly
their function, by S-nitrosylation. These results shed light on the signaling events controlling Cd2+ cytotoxicity in plants.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
R. De Michele, E. Vurro, C. Rigo, A. Costa, L. Elviri, M. Di Valentin, M. Careri, M. Zottini, L.S. di Toppi, F. Lo Schiavo
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