H2O2 in plant peroxisomes : an in vivo analysis uncovers a Ca2+-dependent scavenging system
Articolo
Data di Pubblicazione:
2010
Citazione:
H2O2 in plant peroxisomes : an in vivo analysis uncovers a Ca2+-dependent scavenging system / A. Costa, I. Drago, S. Behera, M. Zottini, P. Pizzo, J.I. Schroeder, T. Pozzan, F. Lo Schiavo. - In: PLANT JOURNAL. - ISSN 0960-7412. - 62:5(2010 Feb), pp. 760-772. [10.1111/j.1365-313X.2010.04190.x]
Abstract:
Oxidative stress is a major challenge for all cells living in an oxygen-based world. Among reactive oxygen
species, H2O2, is a well known toxic molecule and, nowadays, considered a specific component of several
signalling pathways. In order to gain insight into the roles played by H2O2 in plant cells, it is necessary to have a
reliable, specific and non-invasive methodology for its in vivo detection. Hence, the genetically encoded H2O2
sensor HyPer was expressed in plant cells in different subcellular compartments such as cytoplasm and
peroxisomes. Moreover, with the use of the new green fluorescent protein (GFP)-based Cameleon Ca2+
indicator, D3cpv–KVK–SKL, targeted to peroxisomes, we demonstrated that the induction of cytoplasmic Ca2+
increase is followed by Ca2+ rise in the peroxisomal lumen. The analyses of HyPer fluorescence ratios were
performed in leaf peroxisomes of tobacco and pre- and post-bolting Arabidopsis plants. These analyses
allowed us to demonstrate that an intraperoxisomal Ca2+ rise in vivo stimulates catalase activity, increasing
peroxisomal H2O2 scavenging efficiency.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Calcium; Cameleon; Hydrogen peroxide; HyPer; Plant peroxisomes
Elenco autori:
A. Costa, I. Drago, S. Behera, M. Zottini, P. Pizzo, J.I. Schroeder, T. Pozzan, F. Lo Schiavo
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