Data di Pubblicazione:
2004
Citazione:
Potassium and carrot embryogenesis : Are K+ channels necessary for development? / A. Costa, A. Carpaneto, S. Varotto, E. Formentin, O. Marin, E. Barizza, M. Terzi, F. Gambale, F. Lo Schiavo. - In: PLANT MOLECULAR BIOLOGY. - ISSN 0167-4412. - 54:6(2004 Apr), pp. 837-852.
Abstract:
The expression pattern of the KDC1 gene, coding for an inwardly-rectifying K+ channel of Daucus carota,
is described in several embryo stages and seedling tissues. Relative quantitative RT-PCR experiments
indicated that, during (somatic) embryonic development, the KDC1 transcript appears as early as the
globular stage and that the transcript level remains constant throughout the successive heart and torpedo
stages. Thereafter, the KDC1 transcript is preferentially expressed in plant roots, but is also present in other
tissues, and in particular, in the shoot apical meristem. In situ hybridisation experiments showed that in
embryos KDC1 mRNA is detectable preferentially in protoderm cells with a stage dependent expression
pattern. At later times, the hybridisation signal is particularly evident in root hairs, root epidermis and
endodermis, but is also observed in single cell layers corresponding to L1 of the shoot apical meristem and
leaf primordia. Promoter studies with the b-glucuronidase reporter gene confirm preferential expression of
KDC1 in embryo protoderm cells and in plant root epidermis and root hairs. Western blot analysis of
embryonic proteins and immunolocalisation experiments on somatic embryos sections revealed the presence
of KDC1 during embryo development. Consistent with these observations, patch-clamp experiments
performed on protoplasts isolated from embryos at the torpedo stage demonstrated the presence of
functional inward rectifying K+ channels. This is the first report on the expression of a plant ion channel
during embryo development.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Carrot somatic embryogenesis; Embryonic protoderm marker; Plant ion channel; Potassium currents in embryo protoplasts
Elenco autori:
A. Costa, A. Carpaneto, S. Varotto, E. Formentin, O. Marin, E. Barizza, M. Terzi, F. Gambale, F. Lo Schiavo
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