Data di Pubblicazione:
2011
Citazione:
Characterization and differentiation of equine tendon-derived progenitor cells / A.B. Lovati, B. Corradetti, A. Lange Consiglio, C. Recordati, E. Bonacina, D. Bizzaro, F. Cremonesi. - In: JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS. - ISSN 0393-974X. - 25:2 suppl.(2011), pp. S75-S84.
Abstract:
Mesenchymal stem cells have been recently investigated for their potential use in regenerative medicine.
Population of adult stem cells were recently identified in human and lab animal tendons, but no detailed investigations have been made in the equine species. The aim of our study is to identify a progenitor cell population from tendon tissue (TSPCs) in the horse superficial digital flexor tendon that are able to be highly clonogenic, to
grow fast and to differentiate in different induced cell lineages as well as bone marrow derived progenitor cells (BM-MSCs). The hypothesis that TSPCs possess a mesenchymal stem cell behavior opens a new prospective
for tendon regenerative medicine approaches. TSPCs were expanded more rapidly and showed higher plating efficiency when compared with BM-MSCs. Both cell lines expressed identical stem cell markers in vitro and they
were able to differentiate towards osteogenic and adipogenic lineages as demonstrated with cytochemical staining
and mRNA gene expression. TSPCs showed a positive but limited chondrogenic differentiation compared with
BM-MSCs as demonstrated by histological and biochemical analyses. According to our results, equine TSPCs have
high clonogenic properties and proliferating potential, they express stem cell markers and have the capability to
be multipotent as well as BM-MSCs. These findings suggest that TSPCs may represent a good model for stem cell
biology and could be useful for future tendon regenerative medicine investigations.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
A.B. Lovati, B. Corradetti, A. Lange Consiglio, C. Recordati, E. Bonacina, D. Bizzaro, F. Cremonesi
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