Data di Pubblicazione:
2011
Citazione:
Studing translation in Arabidopsis chloroplasts / P. Pesaresi - In: Chloroplast Research in Arabidopsis : Methods and Protocols. 1 / [a cura di] R.P. Jarvis. - New York : Humana press, 2011. - ISBN 9781617792335. - pp. 209-224
Abstract:
Chloroplasts as descendents of a cyanobacterial endosymbiont have retained, during evolution, their own
genome together with the gene expression machinery, including the translation apparatus. Therefore,
chloroplast protein synthesis is not only a key process in organelle biogenesis and maintenance, but it also
represents the major regulatory step in chloroplast gene expression. In fact, several independent evidences
have shown that the accumulation of template messengers is not limiting in the expression of chloroplast
genes. On the contrary, translation regulatory processes based on selection of translatable mRNA by either
nucleus-encoded activation factors or sensors of the assembly status of chloroplast multiprotein complexes
have been reported. Additionally, we have shown that organelle translation rate triggers an organelleto-
nucleus signaling cascade aimed to modulate nuclear gene expression according to the organelle’s
needs. Therefore, the study of chloroplast translation appears to be essential for the comprehension of
several aspects of chloroplast activity. Here, we describe the in vivo pulse-chase and the polysome isolation
approaches. Taken together, the two methods allow one to assess rates of protein synthesis and degradation
as well as defects during the initial steps of protein synthesis.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
Chloroplast ; In vivo labeling ; Protein translation rate , Polysome assembly
Elenco autori:
P. Pesaresi
Link alla scheda completa:
Titolo del libro:
Chloroplast Research in Arabidopsis : Methods and Protocols. 1