Data di Pubblicazione:
2011
Citazione:
Comparative study of fecal calprotectin (FC) rapid test with the established ELISA method / A. Dolci, L. Scapellato, M. Panteghini. - In: CLINICAL CHEMISTRY AND LABORATORY MEDICINE. - ISSN 1434-6621. - 49:Suppl.1(2011), pp. S672-S672. ((Intervento presentato al convegno IFCC - WordLab - EuroMedLab tenutosi a Berlin nel 2011 [10.1515/CCLM.2011.525].
Abstract:
Background. FC is a useful marker for diagnosing inflammatory bowel disease (IBD). However, no automated assay is available
yet and widely adopted ELISA methods show turnaround time of≥4h. We compared the point of care (POC) Quantum Blue FCimmunochromatographic
test (Buhlmann) to the Calprest ELISA (Eurospital) routinely used in our laboratory.
Methods. Stool specimens of 67 consecutive patients were analyzed after extraction using recommended device. FC was determined
on fresh samples by POC, while for the ELISA stool samples were frozen at -20 °C, and thawed, extracted, and assayed
within 2 weeks from collection. We first compared results by Passing Bablok regression and then estimated diagnostic agreement
by adopting 90 μg/g feces as cut-off for ELISAand an experimentally recalculated cut-off for POC.
Results. Method comparison, performed on 28 paired results higher than the POCdetection limit(30 μg/g), showed a slope of 2.24
(ELISA in x-axis), with not significant intercept. On the basis of this finding, the POC cut-off was established at 200μg/g. FC was
positive on 20 samples by ELISA and on 17 samples by POC, with a 92.5% agreement. Among fivepatients showing discrepant
results, 4 were positive on ELISA (two borderline – 94 and 98 μg/g – results) and one positive on POC. This POC “false-positive”
related to a 16-years old girl, with chronic diarrhea but no IBD diagnosis.
Conclusions. Our preliminary results suggest that POC may replace the standard ELISA and make FC testing more rapid (<20
min), effective, and suitable for any laboratory setting.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
A. Dolci, L. Scapellato, M. Panteghini
Link alla scheda completa: