Gene expression profiling reveals multiple differences in platelets from patients with stable angina or NON-ST elevation acute coronary syndrome
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Data di Pubblicazione:
2010
Citazione:
Gene expression profiling reveals multiple differences in platelets from patients with stable angina or NON-ST elevation acute coronary syndrome / M. Brambilla, G. Colombo, K. Gertow, G.C. Marenzi, M. De Metrio, E. Tremoli, M. Camera. ((Intervento presentato al convegno Next Step: la giovane ricerca avanza tenutosi a Milano nel 2010.
Abstract:
Background. Platelets play a key role in coronary artery disease. They have the capacity of protein synthesis through translation of megakaryocyte-derived mRNAs, which may influence pathophysiological functions. Aim. The aim of this study was to identify genes that may modulate the platelet prothrombotic potential in non-ST elevation acute coronary syndrome (NSTE-ACS). Methods. Gene expression profiles were determined in RNA pools from resting platelets of patients with stable angina (SA, n = 14) or NSTE-ACS (n = 15) using a glass microarray platform. Validation was done by real-time PCR and immunoblot analyses in independent sets of individual samples (26 SA and 17 NSTE-ACS patients, in total). Parallel comparison with healthy subjects was performed to relate the relative abundance of validated genes in CAD patients to a control expression level. Results. Microarray analysis identified 45 transcripts with a significant ≥ ±2.0-fold difference in expression between NSTE-ACS and SA platelet pools. Validation confirmed a significant over-expression of 4 transcripts (BAI1-associated protein-2, BAIAP2; clathrin light chain, CLTA; glycoprotein Ib β-chain, GP1BB; and protein kinase inhibitor-γ, PKIG) in NSTE-ACS compared to SA platelets. BAIAP2 and CLTA proteins were more abundant in NSTE-ACS than in SA platelets, whereas GP1BB was increased in NSTE-ACS also in comparison to healthy subjects. Conclusions. Our data identified a set of differentially expressed genes in NSTE-ACS platelets, both at mRNA and protein level, which may modulate platelet reactivity in this pathological condition. These findings support the hypothesis of an intrinsic increased prothrombotic potential of platelets in NSTE-ACS.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Keywords:
Acute coronary syndrome ; platelets ; thrombosis ; gene expression profiling
Elenco autori:
M. Brambilla, G. Colombo, K. Gertow, G.C. Marenzi, M. De Metrio, E. Tremoli, M. Camera
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