Human carotid plaque composition: Correlation between histology and Multidetector computed tomography (MDCT)
Poster
Data di Pubblicazione:
2010
Citazione:
Human carotid plaque composition: Correlation between histology and Multidetector computed tomography (MDCT) / L. Facchinetti, R. Spirito, G. Ballerini, V. Bona, A. Formenti, M. Fusari, E. Tremoli, M. Camera. - In: BLOOD TRANSFUSION. - ISSN 1723-2007. - 8:Suppl. 4(2010), pp. s81-s82. ((Intervento presentato al 21. convegno Congresso Nazionale SISET tenutosi a Bologna nel 2010.
Abstract:
Background Plaque morphology is an important predictor of
stroke risk. Although plaque morphology is not used, so far, in
the decision making of whether to perform carotid
endoarterectomy (CEA) or not, having a non-invasive
technique capable of diagnosing vulnerable plaque would be
very useful in the recognition of patients at high risk of
cerebrovascular disease.
Aim To compare human carotid plaques composition obtained
by means of histological analysis and MDCT.
Methods 37 patients (22 male, 15 females; mean age: 70±7 years)
undergoing CEA due to high grade carotid artery stenosis
(82±9%) were evaluated with MDCT for non-invasive plaque
morphology assessment prior to CEA. Plaques removed during
surgery were divided into 5 mm pieces. The part corresponding to
the point of maximum stenosis was formalin fixed and paraffin
embedded for histology. Sections were stained with
Haematoxilin-Eosin and Masson-Trichrome to assess plaque
morphology and composition (collagen, smooth muscle cell,
lipids and calcium). Kappa statistics was used for the degree of
agreement between the histological and MDTC images.
Results Images of histological sections were computerreconstructed
with Zeiss-Panorama software after being
photographed under a microscope (90 frames for each section on
average). The area occupied by collagen, smooth muscle cells,
lipids, calcium and thrombus was then quantified (absolute
amount, mm2, and percentage over the total area) using the Zeiss-
Axiovision-measurement software. According to the composition
thus obtained, plaques were classified into four classes: lipidic
(10%), fibrotic (45%), mixed (35%), and calcific (10%). DSCT
plaque analysis was performed on a image of the vessel area at the
highest degree of stenosis. A good correlation between histology
and MDCT was found (k=0.76).
Conclusion MDCT angiography of the carotid arteries is
feasible and the evaluation of carotid plaque composition
allows non-invasive assessment of different plaque
components. This may have an impact on the non-invasive
differentiation of vulnerable plaques.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
human carotid plaque ; histology ; Multidetector computed tomography (MDCT)
Elenco autori:
L. Facchinetti, R. Spirito, G. Ballerini, V. Bona, A. Formenti, M. Fusari, E. Tremoli, M. Camera
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