Data di Pubblicazione:
2008
Citazione:
Viability-Dependent Promoting Action of Adult Neural Precursors in Spinal Cord Injury / D. Bottai, L. Madaschi, A.M. Di Giulio, A. Gorio. - In: MOLECULAR MEDICINE. - ISSN 1076-1551. - 14:9-10(2008 Sep), pp. 634-644.
Abstract:
The aim of the study was the assessment of the effects of adult neural stem cell (NSC) transplantation in a mouse model of
spinal cord injury (SCI). The contusion injury was performed by means of the Infinite Horizon Device to allow the generation of reproducible
traumatic lesion to the cord.We administered green fluorescent-labeled (GFP-)NSCs either by intravenous (i.v.) injection
or by direct transplantation into the spinal cord (intraspinal route).We report that NSCs significantly improved recovery of hind
limb function and greatly attenuated secondary degeneration. The i.v. route of NSC administration yielded better recovery than
the intraspinal route of administration. About 2% of total i.v.-administered NSCs homed to the spinal cord injury site, and survived
almost undifferentiated; thus the positive effect of NSC treatment cannot be ascribed to damaged tissue substitution. The NSCs
homing to the injury site triggered, within 48 h, a large increase of the expression of neurotrophic factors and chemokines. One
wk after transplantation, exogenous GFP-NSCs still retained their proliferation potential and produced neurospheres when recovered
from the lesion site and cultured in vitro. At a later time,GFP-NSC were phagocytated by macrophages.We suggest that the
process of triggering the recovery of function might be strongly related to the viability of GFP-NSC, still capable ex vivo of producing
neurospheres, and their ability to modify the lesion environment in a positive fashion
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Spinal cord injury ; neural stem cells ; inflammation ; Real time PCR
Elenco autori:
D. Bottai, L. Madaschi, A.M. Di Giulio, A. Gorio
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