Evaluation of the bacterial microflora of the conjunctival sac of healthy dogs and dogs with atopic dermatitis
Articolo
Data di Pubblicazione:
2011
Citazione:
Evaluation of the bacterial microflora of the conjunctival sac of healthy dogs and dogs with atopic dermatitis / N. Furiani, F. Scarampella, P.A. Martino, I. Panzini, E. Fabbri, L. Ordeix. - In: VETERINARY DERMATOLOGY. - ISSN 0959-4493. - 22:6(2011 Dec), pp. 490-496.
Abstract:
The aim of this case–control study was to evaluate and compare the bacterial microflora from the conjunctival sac of dogs with atopic dermatitis and healthy dogs. Twenty-one atopic dogs without clinical and/or cytopathological signs of bacterial
blepharoconjunctivitis and 21 breed-matched
healthy dogs were enrolled. Under topical anaesthesia, the inferior conjunctival sac of one eye was scraped twice. Material was collected with a Kimura spatula, spread over a slide and stained with a Diff Quick -type stain (Medion Diagnostics GmbH,
Du¨ dingen, Switzerland) for cytological examination.
An area of 0.5 cm2 was examined at ·1000 magnification, and the types and numbers of cells and bacteria were recorded. A bacterial swab was collected and inoculated into culture media for the growth of aerobic bacteria. Before sampling, each atopic dog was evaluated for severity of cutaneous
lesions, pruritus and conjunctival inflammation. Significant differences were observed between atopic and healthy dogs for the presence of bacteria on cytology (P = 0.015), keratinized (P = 0.001) and nonkeratinized epithelial cells (P = 0.013), eosinophils(P = 0.019) and lymphocytes (P = 0.008). Bacteria were recovered from 12 atopic dogs and three healthy dogs (P = 0.004). Staphylococcus pseudintermedius
was the most commonly isolated species in atopic dogs (seven of 12). In atopic
dogs, no significant relation was found between conjunctival bacterial colonization (on cytology and culture) and the severity of any of the clinical parameters. This study suggests differences in conjunctival bacterial colonization and cytological
features between atopic and healthy dogs.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
clinically normal dogs; acvd task-force; staphylococcus-intermedius; malassezia-pachydermatis; ulcerative keratitis; ocular complications; skin; microorganisms; colonization; disease
Elenco autori:
N. Furiani, F. Scarampella, P.A. Martino, I. Panzini, E. Fabbri, L. Ordeix
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