A Selective, Nontoxic, OFF–ON Fluorescent Molecular Sensor Based on 8-Hydroxyquinoline for Probing Cd2+ in Living Cells
Articolo
Data di Pubblicazione:
2010
Citazione:
A Selective, Nontoxic, OFF–ON Fluorescent Molecular Sensor Based
on 8-Hydroxyquinoline for Probing Cd2+ in Living Cells / M. Mameli, M.C. Aragoni, M. Arca, C. Caltagirone, F. Demartin, G. Farruggia, G. De Filippo, F.A. Devillanova, A. Garau, F. Isaia, V. Lippolis, S. Murgia, L. Prodi, A. Pintus, N. Zaccheroni. - In: CHEMISTRY-A EUROPEAN JOURNAL. - ISSN 0947-6539. - 16:3(2010), pp. 919-930.
Abstract:
In spite of the fact that cadmium(
II) has been recognized as a
highly toxic element and that excessive
exposure to this metal ion has been reported
to have many adverse effects on
human health, very few selective and
specific fluorescent probes are available
for imaging Cd2+ in living cells.
Herein, we report the spectroscopic
and photochemical characterization of
5-(5-chloro-8-hydroxyquinolinylmethyl)-
2,8-dithia-5-aza-2,6-pyridinophane
(L) as a fluorescent sensor for the selective
imaging of Cd2+ in living cells.
In particular, the response of L to Cd2+
was first assessed in aqueous solutions,
sodium dodecyl sulfate micelles, and
liposomes, and subsequently in living
cells by fluorescence microscopy techniques.
Cytofluorimetric analyses of
leukemic HL-60 cells loaded with L
also allowed evaluation of the toxicity
of the probe and the selective analysis
of its intracellular fluorescence in the
presence of Cd2+. Furthermore, the 1:1
complex species [Cd(L)H2O]2+ responsible
for the OFF–ON chelation enhancement
of fluorescence (CHEF)
effect on L was structurally characterized;
time-dependent DFT calculations
allowed the prediction of theoretical
excitations, which were comparable
with the experimental ones.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
cadmium ; density functional
calculations ; fluorescent
probes ; imaging agents ; macrocycles
Elenco autori:
M. Mameli, M.C. Aragoni, M. Arca, C. Caltagirone, F. Demartin, G. Farruggia, G. De Filippo, F.A. Devillanova, A. Garau, F. Isaia, V. Lippolis, S. Murgia, L. Prodi, A. Pintus, N. Zaccheroni
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