Structural and functional alterations of the cell nucleus in skeletal muscle wasting : the evidence in situ
Articolo
Data di Pubblicazione:
2010
Citazione:
Structural and functional alterations of the cell nucleus in skeletal muscle wasting : the evidence in situ / M. Malatesta, G. Meola. - In: EUROPEAN JOURNAL OF HISTOCHEMISTRY. - ISSN 1121-760X. - 54:4(2010), pp. e44-e44. [10.4081/ejh.2010.e44]
Abstract:
Myotonic dystrophies (DMs) are characterised
by highly variable clinical manifestations consisting of muscle weakness and atrophy, and a wide spectrum of extramuscular manifestations. In both DM1 and DM2 forms, expanded nucleotide sequences cause the accumulation of mutant transcripts in the nucleus, thus deregulating the function of some RNA-binding proteins and providing a plausible explanation for the multifactorial phenotype of DM patients. However, at the skeletal muscle level, no mechanistic explanation for the muscle wasting has so far been proposed. We therefore performed a study in situ by immunoelectron microscopy on biceps brachii biopsies from DM1, DM2 and healthy subjects, providing the first ultrastructural evidence on the distribution of some nuclear ribonucleoprotein (RNP)-containing structures and molecular factors involved in pre-mRNA transcription and
maturation in dystrophic myonuclei. Our results demonstrated an accumulation of splicing and cleavage factors in myonuclei of both DM1 and DM2 patients, suggesting an impairment of post-transcriptional pre-mRNA pathways. The transcription of the expanded sequences in DM myonuclei would therefore hamper functionality of the whole splicing machinery, slowing down the intranuclear molecular trafficking; this would reduce the capability of myonuclei to respond to anabolic stimuli thus contributing to muscle wasting.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cell nucleus; Histochemistry; Laminopathies; Myotonic dystrophy; Sarcopenia; Skeletal muscle
Elenco autori:
M. Malatesta, G. Meola
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