Identification and structure of the mating-type locus and development of PCR-based markers for mating type in powdery mildew fungi
Articolo
Data di Pubblicazione:
2011
Citazione:
Identification and structure of the mating-type locus and development of PCR-based markers for mating type in powdery mildew fungi / T.M. Brewer, L. Cadle-Davidson, P. Cortesi, P. Spanu, M.G. Milgroom. - In: FUNGAL GENETICS AND BIOLOGY. - ISSN 1087-1845. - 48:7(2011 May), pp. 704-713. [10.1016/j.fgb.2011.04.004]
Abstract:
In ascomycetes, mating compatibility is regulated by the mating-type locus, MAT1. The objectives of this
study were to identify and sequence genes at the MAT1 locus in the grape powdery mildew fungus,
Erysiphe necator, to develop a PCR-based marker for determining mating type in E. necator, and to develop
degenerate primers for amplification by PCR of conserved regions of mating-type idiomorphs in other
powdery mildew fungi. We identified MAT1-2-1 of the MAT1-2 idiomorph in E. necator based on the
homologous sequence in the genome of Blumeria graminis f. sp. hordei and we found MAT1-1-1 and
MAT1-1-3 of the MAT1-1 idiomorph from transcriptome sequences of E. necator. We developed and
applied a reliable PCR-based multiplex marker to confirm that genotype correlated with mating phenotype,
which was determined by pairing with mating-type tester isolates. Additionally, we used the marker
to genotype populations of E. necator from different Vitis spp. from throughout the USA. We found
both mating types were present in all populations and mating-type ratios did not deviate from 1:1.
The mating-type genes in E. necator are similar to those of other Leotiomycetes; however, the structure
of the MAT1 locus in E. necator, like the MAT1-2 idiomorph of B. graminis, is markedly different from other
ascomycetes in that it is greatly expanded and may contain a large amount of repetitive DNA. As a result,
we were unable to amplify and sequence either idiomorph in its entirety. We designed degenerate primers
that amplify conserved regions of MAT1-1 and MAT1-2 in E. necator, Podosphaera xanthii, Microsphaera
syringae, and B. graminis, representing the major clades of the Erysiphales. These degenerate primers or
sequences obtained in this study from these species can be used to identify and sequence MAT1 genes or
design mating-type markers in other powdery mildew fungi as well.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Erysiphales; Erysiphe necator; MAT locus; Mating type; Powdery mildew; Transcriptome
Elenco autori:
T.M. Brewer, L. Cadle Davidson, P. Cortesi, P. Spanu, M.G. Milgroom
Link alla scheda completa: