Analysis of histological and immunohistochemical pattern of hepatocellular carcinoma expressing cytokeratin 7 by computerized morphometry
Poster
Data di Pubblicazione:
2011
Citazione:
Analysis of histological and immunohistochemical pattern of hepatocellular carcinoma expressing cytokeratin 7 by computerized morphometry / M. Vertemati, E. Minola, C. Moscheni, D. Petrella, M. Cossa, M. Goffredi, L. Vizzotto. - In: JOURNAL OF HEPATOLOGY. - ISSN 0168-8278. - 54:Suppl. 1(2011 Mar), pp. S405-S405. (Intervento presentato al 46. convegno Annual meeting of the European Association for the Study of the Liver tenutosi a Berlin nel 2011) [10.1016/S0168-8278(11)61021-0].
Abstract:
Background and Aims: Recent studies have identified a subset
of HCC containing cells expressing cytokeratin 7 (CyK7). These
CyK7 positive cells potentially originates from hepatic progenitor
cells and are activated in chronic liver disease. Furthermore, they
could give rise to HCC. Our aim was to apply a computerized
morphometric model to evaluate and quantify the morphologic
features, including hepatic progenitor cells, in hepatocellular
carcinoma (HCC) expressing CyK7.
Methods: This study included 12 HCC expressing CyK7 from
12 patients submitted to LTx for HCV-related cirrhosis at the
Niguarda Hospital in Milan. For each case, five consecutive sections
4 mm thick were obtained from the selected block. All specimens
were stained with hematoxylin-eosin and immunohistochemically
for CD34 to assess the degree of capillarization and reticulin to
assess the cell plate architecture. Moreover, we have done a dual
immunohistochemical labeling with CyK7 and Ki67 to identify
respectively neoplastic cells expressing biliary marker and tumor
cells that are actively dividing. We determined by a computerized
morphometric model the volume fractions occupied by hepatocyte
nuclei and cytoplasm, sinusoids, portal triads, capillarised sinusoids,
tumor cells respectively expressing biliary marker and actively
dividing. Lastly, the surface fraction occupied by reticulin was
calculated. Results have been compared with a control group of
10 high-grade dysplastic nodules (DN) from 10 patients submitted
to LTx for HCV-related cirrhosis.
Results: Volume fractions of capillarised sinusoids, CyK7 positive
cells and Ki67 positive cells were more prominent in HCC when
compared to DN; surface fraction of reticulin was markedly
decreased in HCC. Moreover, in HCC the dual immunohistochemical
labeling performed for CyK7 and Ki67 identified a group of cells
expressing both immunostains: the number of these cells correlated
with both the number of cells expressing CyK7psitive and Ki67
positive (p < 0.0001). Other features most discriminatory between
DN and HCC were: volume fractions occupied by hepatocytes nuclei
(p < 0.0001) and cytoplasm (p < 0.001), nuclear/cytoplasmic ratio
(p < 0.0001).
Conclusion: Our morphometric model is an objective method of
quantification of the morphologic changes in HCC expressing CyK7;
moreover, cells expressing both immunostains (CyK7/Ki67) could
represent cells in continuous proliferation potentially derived from
hepatic progenitors cells.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Morphometry; Cytokeratine; Hepatocellular Carcinoma
Elenco autori:
M. Vertemati, E. Minola, C. Moscheni, D. Petrella, M. Cossa, M. Goffredi, L. Vizzotto
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