Data di Pubblicazione:
2010
Citazione:
Evaluation of semi-quantitative results of the CMV DBS-test / A. Mammoliti, S. Binda, L. Bubba, M. Gambino, L. Pellegrinelli, V. Primache, C. Pietrasanta, C. Corbetta, M. Barbi. ((Intervento presentato al 3. convegno Congenital Cytomegalovirus Conference tenutosi a Paris nel 2010.
Abstract:
BRIEF INTRODUCTION
Quantification of CMV-DNA may identify children at higher risk of late sequelae of congenital CMV
infection (cCMV). In this study we investigated the relation between semi-quantitative and
quantitative results obtained in testing neonatal DBS, in the aim of using the CMV DBS-test for a
preliminary formulation of prognosis.
MATERIALS AND METHODS
DBS of 232 newborns with isolation confirmed cCMV were analyzed by means of: a) DBS-test (Barbi et
al., 2006). DNA purification: thermal shock. Amplification: nested-PCR (target: gB gene). Positive
results were distinguished in “weak” (with 1/3 positive tests) and “strong” (at least 2/3). b)
Quantitative Real-time PCR, “Q-CMV Real-time Complete Kit”, Nanogen (MIEA gene). DNA extraction:
“QIAamp DNA Mini-kit” (QIAGEN). Clinical data available for 148 children at birth and follow-up were
correlated to the semi-quantitative results.
CLINICAL CASES OR SUMMARY RESULTS
DBS-test results were “strong” in 197 cases, “weak” in 32 and negative in 3. Real-time PCR was
positive in 184 cases (mean: 9,228; median: 1,278, range: 9-897,876 copies/ml) and negative in 48.
“Strong” cases had median viral loads higher than “weak” cases (1,447 vs 702 copies/ml;
Mann-Whitney’s Test, p<0.01). Babies symptomatic at birth were 30% in the “weak” group and 51% in
the “strong” one. Seventeen out of 74 DBS (23%) with a “strong” positivity but none of 12 “weak” cases
developed SNHL.
CONCLUSIONS
This study highlights the importance of testing DBS following a suitable algorithm in order of not
missing cases with a low viral load. The confirmation of results will permit to have preliminary
indications on prognosis, merely applying a method easy and cheap as the DBS-test.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Keywords:
Cytomegalovirus ; CMV ; DBS ; dried blood spots ; DBS-test ; congenital infection
Elenco autori:
A. Mammoliti, S. Binda, L. Bubba, M. Gambino, L. Pellegrinelli, V. Primache, C. Pietrasanta, C. Corbetta, M. Barbi
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