Data di Pubblicazione:
2004
Citazione:
The anticoagulant thrombin mutant W215A/E217A has a collapsed primary specificity pocket / A.O. Pineda, Z.W. Chen, S. Caccia, A.M. Cantwell, S.N. Savvides, G. Waksman, F.S. Mathews, E. Di Cera. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 279:38(2004 Sep 17), pp. 39824-39828.
Abstract:
The thrombin mutant W215A/E217A features a drastically impaired catalytic activity toward chromogenic and natural substrates but efficiently activates the anticoagulant protein C in the presence of thrombomodulin. As the remarkable anticoagulant properties of this mutant continue to be unraveled in preclinical studies, we solved the x-ray crystal structures of its free form and its complex with the active site inhibitor H-d-Phe-Pro-Arg-CH(2)Cl (PPACK). The PPACK-bound structure of W215A/E217A is identical to the structure of the PPACK-bound slow form of thrombin. On the other hand, the structure of the free form reveals a collapse of the 215-217 strand that crushes the primary specificity pocket. The collapse results from abrogation of the stacking interaction between Phe-227 and Trp-215 and the polar interactions of Glu-217 with Thr-172 and Lys-224. Other notable changes are a rotation of the carboxylate group of Asp-189, breakage of the H-bond between the catalytic residues Ser-195 and His-57, breakage of the ion pair between Asp-222 and Arg-187, and significant disorder in the 186- and 220-loops that define the Na(+) site. These findings explain the impaired catalytic activity of W215A/E217A and demonstrate that the analysis of the molecular basis of substrate recognition by thrombin and other proteases requires crystallization of both the free and bound forms of the enzyme.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
A.O. Pineda, Z.W. Chen, S. Caccia, A.M. Cantwell, S.N. Savvides, G. Waksman, F.S. Mathews, E. Di Cera
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