Synthesis, chiral resolution, and enantiopharmacology of a potent 2,3-benzodiazepine derivative as noncompetitive AMPA receptor antagonist
Articolo
Data di Pubblicazione:
2006
Citazione:
Synthesis, chiral resolution, and enantiopharmacology of a potent 2,3-benzodiazepine derivative as noncompetitive AMPA receptor antagonist / M. Zappalà, G. Postorino, N. Micale, S. Caccamese, N. Parrinello, G. Grazioso, G. Roda, F.S. Menniti, G. De Sarro, S. Grasso. - In: JOURNAL OF MEDICINAL CHEMISTRY. - ISSN 0022-2623. - 49:2(2006), pp. 575-581. [10.1021/jm050552y]
Abstract:
This paper describes the synthesis of racemic 3,5-dihydro-5-methyl-7,8-methylenedioxy-4H-2,3-benzodiazepin-4-one (+/-)-5, attempted stereoselective synthesis of its enantiomers, chiral HPLC resolution of the racemate, and assignment of the absolute configuration. Enantiomer (5S)-(-)-5 is provided with an in vivo anticonvulsant activity 8 times higher than its enantiomer (5R)-(+)-5. This result is confirmed in the in vitro test by the ability to inhibit the kainate-induced increase of the [Ca2+](i) in a primary culture of rat cerebellar granule cells which express alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors. Binding affinity of compound ()-5 at the AMPA and N-methyl-D-aspartic acid (NMDA) receptors was also evaluated.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Absolute-configuratin; semiempirical methods; MK-801 binding; conformations; optimization; parameters; molecules; continuum; seizures; brain
Elenco autori:
M. Zappalà, G. Postorino, N. Micale, S. Caccamese, N. Parrinello, G. Grazioso, G. Roda, F.S. Menniti, G. De Sarro, S. Grasso
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