TLQP-21, a VGF-derived peptide, prevents ethanol-induced gastric lesions: insights into its mode of action
Articolo
Data di Pubblicazione:
2010
Citazione:
TLQP-21, a VGF-derived peptide, prevents ethanol-induced gastric lesions: insights into its mode of action / V. Sibilia, F. Pagani, I. Bulgarelli, E. Mrak, M. Broccardo, G. Improta, C. Severini, R. Possenti, F. Guidobono. - In: NEUROENDOCRINOLOGY. - ISSN 0028-3835. - 92:3(2010), pp. 189-197. [10.1159/000319791]
Abstract:
BACKGROUND AND AIM:
TLQP-21, a peptide derived from the vgf gene, has been reported to play a role in the regulation of rat gastric motility, but its influence on gastric mucosal integrity is unknown.
EXPERIMENTAL APPROACH:
We investigated the effects of central (0.8-8 nmol/rat) or peripheral (48-240 nmol/kg) TLQP-21 administration on ethanol- (EtOH, 50%, 1 ml/rat) induced gastric lesions in the rat. The mechanisms involved in such activity were also examined.
RESULTS:
Central TLQP-21 injection dose-dependently reduced EtOH-induced gastric lesions (ED(50) = 3.16 nmol), while peripheral TLQP-21 administration had no effect. The TLQP-21 gastroprotective effect against EtOH injury was accompanied by a significant increase in gastric prostaglandin E(2) (PGE(2)) production linked to an increase in constitutive cyclooxygenase (COX) expression. The nitric oxide (NO) synthase inhibitor L-NAME (70 mg/kg, s.c.), the nonselective COX inhibitor indomethacin (10 mg/kg, orally) and capsaicin denervation removed TLQP-21 gastroprotection.
CONCLUSIONS:
This study shows for the first time that central TLQP-21 exerts a protective action on the gastric mucosa exposed to the noxious agent EtOH. TLQP-21 gastroprotection is mediated by constitutive-derived NO and PGE(2), and requires the integrity of sensory nerve fibers.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
TLQP-21 ; Ethanol ; Gastric ulcers ; Prostaglandins ; Nitric oxide ; Sensory fibers
Elenco autori:
V. Sibilia, F. Pagani, I. Bulgarelli, E. Mrak, M. Broccardo, G. Improta, C. Severini, R. Possenti, F. Guidobono
Link alla scheda completa: