Comprehensive proteomic analysis reveals omega-3 fatty acids to counteract endotoxin-stimulated metabolic dysregulation in porcine enterocytes
Articolo
Data di Pubblicazione:
2023
Citazione:
Comprehensive proteomic analysis reveals omega-3 fatty acids to counteract endotoxin-stimulated metabolic dysregulation in porcine enterocytes / T.S. Sundaram, M.F. Addis, C. Giromini, R. Rebucci, S. Pisanu, D. Pagnozzi &, A. Baldi. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 13:(2023 Dec 07), pp. 21595.1-21595.15. [10.1038/s41598-023-48018-3]
Abstract:
Omega-3 polyunsaturated fatty acids (n-3 PUFA), such as the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are reported to beneficially affect the intestinal immunity. The biological pathways modulated by n-3 PUFA during an infection, at the level of intestinal epithelial barrier remain elusive. To address this gap, we investigated the proteomic changes induced by n-3 PUFA in porcine enterocyte cell line (IPEC-J2), in the presence and absence of lipopolysaccharide (LPS) stress conditions using shotgun proteomics analysis integrated with RNA-sequencing technology. A total of 33, 85, and 88 differentially abundant proteins (DAPs) were identified in cells exposed to n-3 PUFA (DHA:EPA), LPS, and n-3 PUFA treatment followed by LPS stimulation, respectively. Functional annotation and pathway analysis of DAPs revealed the modulation of central carbon metabolism, including the glycolysis/gluconeogenesis, pentose phosphate pathway, and oxidative phosphorylation processes. Specifically, LPS caused metabolic dysregulation in enterocytes, which was abated upon prior treatment with n-3 PUFA. Besides, n-3 PUFA supplementation facilitated enterocyte development and lipid homeostasis. Altogether, this work for the first time comprehensively described the biological pathways regulated by n-3 PUFA in enterocytes, particularly during endotoxin-stimulated metabolic dysregulation. Additionally, this study may provide nutritional biomarkers in monitoring the intestinal health of human and animals on n-3 PUFA-based diets.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Omega-3 polyunsaturated fatty acid(s), Docosahexaenoic acid, Eicosapentaenoic acid, Intestinal epithelial cells, Inflammation, Proteomics;
Elenco autori:
T.S. Sundaram, M.F. Addis, C. Giromini, R. Rebucci, S. Pisanu, D. Pagnozzi &, A. Baldi
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