First evidences for a third sulfatase maturation system in prokaryotes from E. coli aslB and ydeM deletion mutants
Articolo
Data di Pubblicazione:
2007
Citazione:
First evidences for a third sulfatase maturation system in prokaryotes from E. coli aslB and ydeM deletion mutants / B. Benjdia, G. Deho', S. Rabot, O. Berteau. - In: FEBS LETTERS. - ISSN 0014-5793. - 581:5(2007), pp. 1009-1014.
Abstract:
To be active all known arylsulfatases undergo a unique post-translational modification leading to the conversion of an active site residue (serine or cysteine) into a Ca-formylglycine.
Although deprived of sulfatase activity, Escherichia coli K12 can efficiently mature heterologous Cys-type sulfatases.
Three potential enzymes (AslB, YdeM and YidF) belonging to the anaerobic sulfatase maturating enzyme family (an SME) are present in its genome. Here we show that E. coli could mature Cys-type sulfatases only in aerobic conditions and that knocking-out of aslB, ydeM and yidF does not impair Cys-type sulfatase maturation. These findings demonstrate that these putative anSME are not involved in Cys-type sulfatase maturation and strongly support the existence of a second, oxygendependent and Cys-type specific sulfatase maturation system among prokaryotes.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
AnSME; FGE; Formylglycine; Radical AdoMet; Radical SAM; Sulfatase
Elenco autori:
B. Benjdia, G. Deho', S. Rabot, O. Berteau
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