Determination of linezolid in human plasma by high-performance liquid chromatography with ultraviolet detection
Articolo
Data di Pubblicazione:
2010
Citazione:
Determination of linezolid in human plasma by high-performance liquid chromatography with ultraviolet detection / D. Cattaneo, S. Baldelli, F. Conti, V. Cozzi, E.G.I. Clementi. - In: THERAPEUTIC DRUG MONITORING. - ISSN 0163-4356. - 32:4(2010), pp. 520-524. [10.1097/FTD.0b013e3181d5eeee]
Abstract:
A high-performance liquid chromatographic method for the determination of linezolid in human plasma was developed and validated. After precipitation of plasma proteins with perchloric acid, the protein-free supernatant was separated by isocratic reverse-phase chromatography on a X Bridge C18 column. The mobile phase consisted of a mixture of phosphoric acid 0.05%: acetonitrile (75:25, v/v) with a flow rate of 1 mL/min. The column elute was monitored at 254 nm. The method was linear from 0.2 to 48 mg/L (mean r = 0.9996, n = 10). The observed intra-and inter-day assay imprecision ranged from 2.83% to 8.16% (18.80% at the lower limit of quantification); inaccuracy varied between-0.33% and 8.18%. Mean drug recovery was 99.8% for linezolid and 90.0% for the internal standard (para-toluic acid). The method was found to be precise and accurate and suitable for therapeutic drug monitoring of linezolid in routine clinical practice.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
HPLC; human plasma; linezolid; pharmacokinetics; therapeutic drug monitoring
Elenco autori:
D. Cattaneo, S. Baldelli, F. Conti, V. Cozzi, E.G.I. Clementi
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