Morphological and functional features of human omental and subcutaneous adipose-derived stem cells isolated from obese patients
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Data di Pubblicazione:
2010
Citazione:
Morphological and functional features of human omental and subcutaneous adipose-derived stem cells isolated from obese patients / L. Salvatori, F. Caporuscio, L. De Girolamo, D. Stanco, G.F. Silecchia, S. Mariani, L. Principessa, L. Ravenna, C. Lubrano, A.T. Brini, E. Petrangeli. ((Intervento presentato al 92. convegno Endocrine Society Annual Meeting & EXPO (ENDO) tenutosi a San Diego nel 2010.
Abstract:
In the last years, the relevance and the interest in adipose tissues have greatly grown due to the knowledge of the
presence of human Adipose-derived Stem Cells (hASC), which has been shown to differentiate into several cell lineages.
The aim of this study is to characterize adipose progenitor cells subpopulations isolated from subcutaneous or visceral
fat in normal and severe obese patients. The different state in the two kinds of patients could, indeed, influence the
hASC phenotype and thus their proliferation capacity and differentiation potential.
The surgeons performed the harvesting of adipose tissue from subcutaneous and visceral areas, both in 10 normal and
10 obese (BMI> 35 Kg/m2) subjects, who already needed a surgical intervention for different reasons. The fat was
collected and immediately processed to obtain the progenitor cells, as previously described (L De Girolamo et al., 2007).
The experiments have been performed at 4th passage when the cells appeared to form an homogenous population with
similar size and granularity.
The proliferation rate was quite similar in hASC isolated from subcutaneous tissues of normal and obese patients with a
doubling time of 118 ± 61 and 96 ± 7 hours, respectively. However, hASC isolated from visceral tissue of obese patients
showed a very lower rate of proliferation (doubling time of 227 ± 71 hours). Colony formation of the hASC population
was determined by Fibroblast-Colony Forming Unit (CFU-F) assay. The clonogenic potential of hASC obtained from
subcutaneous normal and obese patients was variable but higher than that showed by the hASC isolated from omental
tissues. The immunophenotype of hASC has been analyzed by cytofluorimetry, evaluating the expression of the surface
markers CD13, CD29, CD44, CD54, CD90 e CD105, CD133, CD14, CD34, CD45, CD71 e CD166. The results showed that
the phenotypic profile of hASC was similar in the different tissues.
In conclusions the hASC isolated from normal and obese subcutaneous and visceral tissues showed different biological
behaviours. The gene expression profile of the hASC isolated from visceral and subcutaneous tissues of normal and
obese subjects will be analyzed, as well as their potential to differentiate into osteogenic, chondrogenic and adipogenic
lineages. Defining the molecular and cellular components of obesity offers the foundation for identifying appropriate
targets to ameliorate the management of the obesity.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
L. Salvatori, F. Caporuscio, L. De Girolamo, D. Stanco, G.F. Silecchia, S. Mariani, L. Principessa, L. Ravenna, C. Lubrano, A.T. Brini, E. Petrangeli
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