ANALYSIS OF INHERENT PROPERTIES OF ION CHANNELS FROM PBCV-1 CHLORELLA VIRUS AND INFLUENZA A VIRUS.
Tesi di Dottorato
Data di Pubblicazione:
2011
Citazione:
ANALYSIS OF INHERENT PROPERTIES OF ION CHANNELS FROM PBCV-1 CHLORELLA VIRUS AND INFLUENZA A VIRUS / M.l. Di Francesco ; tutor: Anna Moroni. DIPARTIMENTO DI BIOLOGIA, 2011 Jan 13. 23. ciclo, Anno Accademico 2010. [10.13130/di-francesco-mattia-lorenzo_phd2011-01-13].
Abstract:
Kcv from the chlorella virus PBCV-1 is a viral protein that forms a tetrameric, functional K+ channel in heterologous
systems. Kcv can serve as a model system to study and manipulate basic properties of the K+ channel pore because
its minimalistic structure (94 amino acids) produces basic features of ion channels, such as selectivity, gating,
and sensitivity to blockers. We present a characterization of Kcv properties at the single-channel level. In symmetric
100 mM K+, single-channel conductance is 114 ± 11 pS. Two different voltage-dependent mechanisms are responsible
for the gating of Kcv. “Fast” gating, analyzed by distributions, is responsible for the negative slope conductance
in the single-channel current–voltage curve at extreme potentials, like in MaxiK potassium channels, and can
be explained by depletion-aggravated instability of the filter region. The presence of a “slow” gating is revealed by
the very low (in the order of 1–4%) mean open probability that is voltage dependent and underlies the time-dependent
component of the macroscopic current.
Tipologia IRIS:
Tesi di dottorato
Keywords:
PBCV1-Kcv ; Chlorella virus
Elenco autori:
M.L. DI FRANCESCO
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