Data di Pubblicazione:
2010
Citazione:
Factor XIII - an under diagnosed deficiency - are we using the right assays? / A.S. Lawrie, L. Green, I.J. Mackie, R. Liesner, S.J. Machin, F. Peyvandi. - In: JOURNAL OF THROMBOSIS AND HAEMOSTASIS. - ISSN 1538-7933. - 8:11(2010 Nov), pp. 2478-2482. [10.1111/j.1538-7836.2010.04028.x]
Abstract:
BACKGROUND: The clot solubility test is the most widely used method for detection of factor (F)XIII deficiency. However, it will only detect severe deficiencies; consequently mild deficiencies and heterozygous states are probably under diagnosed.
OBJECTIVE: As an alternative first-line screening test, we assessed an automated quantitative ammonia release assay (QARA).
PATIENTS/METHODS: Inter-assay imprecision was evaluated with commercial normal and pathological control plasmas (10 replicates on each of 5 days). Using the QARA and other commercial assays a comparative assessment of congenital (FXIII range < 1-70 u dL(-1), n = 9) and acquired (n = 43) deficiencies was made. We also investigated the prevalence of acquired deficiencies in hospitalized patients using residual samples from adult patients (n = 1004) and from a paediatric intensive care unit (ICU, n = 56).
RESULTS: Assay imprecision was acceptably low (normal control: mean 86.6 u dL(-1); cv = 2.0%; pathological control: mean 27.5 u dL(-1); cv = 3.8%). Using an iodoacetamide blanking procedure, the QARA results (FXIII range < 1-70 u dL(-1)) exhibited close agreement with those from an immuno-turbidometric FXIII A-subunit (FXIII-A) method. There was also good correlation (R(2) ≥ 0.89) between the QARA (range 20-180 u dL(-1)), a second chromogenic assay, the FXIII-A and FXIII A+B-subunit ELISA. We found that 21% of samples from adult patients had FXIII levels < 70 u dL(-1) (mean normal ± 2 SD 73-161 u dL(-1)) with 6% < 50 u dL(-1). Within the paediatric ICU samples, 52% were < 70 u dL(-1), with 21% < 50 u dL(-1).
CONCLUSIONS: Our data demonstrates that the automated assay is sensitive, highly reproducible and the results from clinical samples suggest that acquired FXIII deficiency is a relatively common phenomenon in hospital patients after surgery and in ICU
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Acquired FXIII deficiency; Factor XIII; Factor XIII deficiency; FXIII assay
Elenco autori:
A.S. Lawrie, L. Green, I.J. Mackie, R. Liesner, S.J. Machin, F. Peyvandi
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