Data di Pubblicazione:
2010
Citazione:
Sphingolipidomics af A2780 human ovarian carcinoma cells treated with synthetic retinoids / M. Valsecchi, M. Aureli, L. Mauri, V.L. Chigorno, A.E.G. Prinetti, S. Sonnino. - In: JOURNAL OF LIPID RESEARCH. - ISSN 0022-2275. - 51:7(2010), pp. 1832-1840. [10.1194/jlr.M004010]
Abstract:
The dihydroceramide, ceramide, sphingomyelin, lactosylceramide, and ganglioside species of A2780 human ovarian carcinoma cells treated with the synthetic retinoids N -(4-hydroxyphenyl)retinamide (fenretinide, 4-HPR) and 4-oxo- N -(4-hydroxyphenyl)retinamide (4-oxo-4-HPR) in culture were characterized by ESI-MS. We characterized 32 species of ceramide and dihydroceramide, 15 of sphingomyelin, 12 of lactosylceramide, 9 of ganglioside GM2, and 6 of ganglioside GM3 differing for the long-chain base and fatty acid structures. Our results indicated that treatment with both 4-HPR and 4-oxo-4-HPR led to a marked increase in dihydroceramide species, while only 4-oxo-4-HPR led to a minor increase of ceramide species. Dihydroceramides generated in A2780 cells in response to 4-HPR or 4-oxo-4-HPR differed for their fatty acid content, suggesting that the two drugs differentially affect the early steps of sphingolipid synthesis.jlr Dihydroceramides produced upon treatments with the drugs were further used for the synthesis of complex dihydrosphingolipids, whose levels dramatically increased in drug-treated cells. Copyright
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
4-HPR; 4-oxo-4-HPR; Fenretinide
Elenco autori:
M. Valsecchi, M. Aureli, L. Mauri, V.L. Chigorno, A.E.G. Prinetti, S. Sonnino
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