Modulation of cell proteome by 25-hydroxycholesterol and 27-hydroxycholesterol: A link between cholesterol metabolism and antiviral defense
Articolo
Data di Pubblicazione:
2020
Citazione:
Modulation of cell proteome by 25-hydroxycholesterol and 27-hydroxycholesterol: A link between cholesterol metabolism and antiviral defense / A. Civra, M. Colzani, V. Cagno, R. Francese, V. Leoni, G. Aldini, D. Lembo, G. Poli.. - In: FREE RADICAL BIOLOGY & MEDICINE. - ISSN 0891-5849. - 149:(2020), pp. 30-36. [10.1016/j.freeradbiomed.2019.08.031]
Abstract:
Physiological cholesterol metabolism implies the generation of a series of oxidized derivatives, whose oxysterols are by far the most investigated ones for their potential multifaceted involvement in human pathophysiology. In this regard, noteworthy is the broad antiviral activity displayed by defined side chain oxysterols, in particular 25-hydroxycholesterol (25HC) and 27-hydroxycholesterol (27HC). Although their antiviral mechanism(s) may vary depending on virus/host interaction, these oxysterols share the common feature to hamper viral replication by interacting with cellular proteins. Here reported is the first analysis of the modulation of a cell proteome by these two oxysterols, that, besides yielding additional clues about their potential involvement in the regulation of sterol metabolism, provides novelinsights about the mechanism underlying the inhibition of virus entry and trafficking within infected cells. We show here that both 25HC and 27HC can down-regulate the junction adhesion molecule-A (JAM-A) and the cation independent isoform of mannose-6-phosphate receptor (MPRci), two crucial molecules for the replication of all those viruses that exploit adhesion molecules and the endosomal pathway to enter and diffuse within target cells.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
25-Hydroxycholesterol; 27-Hydroxycholesterol; Antiviral activity; Oxysterols; SILAC proteomics; Sterol metabolism
Elenco autori:
A. Civra, M. Colzani, V. Cagno, R. Francese, V. Leoni, G. Aldini, D. Lembo, G. Poli.
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