Cytokine mRNA expression in the bronchoalveolar lavage cells from horses affected by different equine asthma subtypes
Articolo
Data di Pubblicazione:
2024
Citazione:
Cytokine mRNA expression in the bronchoalveolar lavage cells from horses affected by different equine asthma subtypes / C.M. LO FEUDO, L. Stucchi, C. Bazzocchi, A. LANGE CONSIGLIO, S. Comazzi, M.C.S. Cozzi, C. Gusmara, G. Gaspari, C. Cialini, D. Bizzotto, R. Dellaca, F. Ferrucci. - In: JOURNAL OF EQUINE VETERINARY SCIENCE. - ISSN 0737-0806. - 135:(2024 Feb 27), pp. 105033.1-105033.7. [10.1016/j.jevs.2024.105033]
Abstract:
Equine asthma (EA) is a respiratory syndrome associated with the increase of different leukocyte populations in
the bronchoalveolar lavage fluid (BALF). Its pathogenetic mechanisms remain unclear. This study aimed to
evaluate the associations between the mRNA expression of different cytokines in the BALF, different EA subtypes
and lung function. Fifteen horses underwent physical examination, airway endoscopy, BALF cytology and lung
function testing (8/15). One horse did not have evidence of EA and was used as healthy reference, while the
others were classified as affected by neutrophilic or mixed granulocytic EA. Cells isolated from the residual BALF
were used for IL-1β, IL-2, IFN-γ, IL-4, IL-17A genes expression by quantitative RT-PCR., Cytokine expression was
compared between groups, and their correlations with BALF leukocyte and lung function were evaluated. IL-1β
expression was positively correlated with BALF neutrophils count (p=0.038, r=0.56) and with increased expi-
ratory resistance (p=0.047, r=0.76). IFN-γ was correlated with BALF mast cells (p=0.029, r=0.58). IL-4 was
higher in horses with mixed granulocytic EA than neutrophilic (p=0.008), positively correlated with BALF mast
cells (p=0.028, r=0.59) and inversely with whole-breath (p=0.046, r=-0.76) and expiratory reactance (p=0.003,
r=-0.93). Finally, IL-17A was inversely correlated with expiratory reactance (p=0.009, r=-0.92). These results
support that multiple immune responses are involved in EA pathogenesis; innate, Th2, and Th17 responses.
Innate immunity appeared associated with neutrophilic inflammation, and Th2 response with increased mast
cells. The role of Th1 response in EA remains questionable.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Asthma immunology; Bronchoalveolar lavage; Cytokine expression; Equine asthma; Horse
Elenco autori:
C.M. LO FEUDO, L. Stucchi, C. Bazzocchi, A. LANGE CONSIGLIO, S. Comazzi, M.C.S. Cozzi, C. Gusmara, G. Gaspari, C. Cialini, D. Bizzotto, R. Dellaca, F. Ferrucci
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