Administration of a synbiotic containing enterococcus faecium does not significantly alter fecal microbiota richness or diversity in dogs with and without food-responsive chronic enteropathy
Articolo
Data di Pubblicazione:
2019
Citazione:
Administration of a synbiotic containing enterococcus faecium does not significantly alter fecal microbiota richness or diversity in dogs with and without food-responsive chronic enteropathy / R. Pilla, B.C. Guard, J.M. Steiner, F.P. Gaschen, E. Olson, D. Werling, K. Allenspach, S.S. Schmitz, J.S. Suchodolski. - In: FRONTIERS IN VETERINARY SCIENCE. - ISSN 2297-1769. - 6:(2019), pp. 277.1-277.9. [10.3389/fvets.2019.00277]
Abstract:
Background: Canine chronic enteropathies (CE) are a group of intestinal diseases that can be categorized based on treatment response into diet- or food- responsive enteropathy (FRD), antibiotic-responsive enteropathy, steroid-responsive enteropathy, and non-responsive enteropathy. CE can often be associated with intestinal dysbiosis and thus administration of probiotic or synbiotic products may provide a useful tool for the management of some of these patients. Enterococcus faecium (EF) is a probiotic strain included in a commercially available synbiotic for small animals, however its impact on the microbial communities in dogs with FRD has not yet been evaluated. Hypothesis/Objectives: The administration of a synbiotic will lead to a significant difference of the fecal microbial composition and/or diversity in dogs with FRD, and these changes are not attributable to diet change alone. Animals/Samples: Twelve dogs with FRD fed a hydrolyzed protein diet received either a synbiotic (EF, fructooligosaccharides, gum Arabic) or placebo. Fecal samples were taken before and 6 weeks into treatment. Fecal samples were also acquired from 8 healthy dogs before and 6 weeks after being switched to the same hydrolyzed protein diet as their sole food. Methods: Bacterial DNA was extracted from fecal samples and next generation sequencing based on the 16S rRNA genes was performed. Microbial composition and diversity between groups were compared using QIIME. Results: There was a small increase in species diversity in the feces of dogs with FRD treated with synbiotics. However, there were no significant differences in microbial community composition before and after 6 weeks in either the synbiotic or placebo treated dogs with FRD or the healthy dogs. In all groups, large individual variations were observed. Conclusions: No changes in microbial composition were observed in diseased or healthy dogs with diet change alone. However, administration of a synbiotic increased bacterial richness in both groups.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Diarrhea; Enterococcus; Inflammatory bowel disease; Microbiome; Probiotics
Elenco autori:
R. Pilla, B.C. Guard, J.M. Steiner, F.P. Gaschen, E. Olson, D. Werling, K. Allenspach, S.S. Schmitz, J.S. Suchodolski
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