Data di Pubblicazione:
2024
Citazione:
Novel loci associated with resistance to downy and powdery mildew in grapevine / V. Ricciardi, M. Crespan, G. Maddalena, D. Migliaro, L. Brancadoro, D. Maghradze, O. Failla, S.L. Toffolatti, G. De Lorenzis. - In: FRONTIERS IN PLANT SCIENCE. - ISSN 1664-462X. - 15:(2024), pp. 1386225.1-1386225.14. [10.3389/fpls.2024.1386225]
Abstract:
Among the main challenges in current viticulture, there is the increasing demand
for sustainability in the protection from fungal diseases, such as downy mildew
(DM) and powdery mildew (PM). Breeding disease-resistant grapevine varieties is
a key strategy for better managing fungicide inputs. This study explores the
diversity of grapevine germplasm (cultivated and wild) from Caucasus and
neighboring areas to identify genotypes resistant to DM and PM, based on 13
Simple Sequence Repeat (SSR) loci and phenotypical (artificial pathogen
inoculation) analysis, and to identify loci associated with DM and PM
resistance, via Genome-Wide Association Analysis (GWAS) on Single
Nucleotide Polymorphism (SNP) profiles. SSR analysis revealed resistant alleles
for 16 out of 88 genotypes. Phenotypic data identified seven DM and 31 PM
resistant genotypes. GWAS identified two new loci associated with DM
resistance, located on chromosome 15 and 16 (designated as Rpv36 and
Rpv37), and two with PM resistance, located on chromosome 6 and 17
(designated as Ren14 and Ren15). The four novel loci identified genomic
regions rich in genes related to biotic stress response, such as genes involved
in pathogen recognition, signal transduction and resistance response. This study
highlights potential candidate genes associated with resistance to DM and PM,
providing valuable insights for breeding programs for resistant varieties. To
optimize their utilization, further functional characterization studies
are recommended.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
GWAS; SSR; SNP; phenotyping; Rpv; Ren; candidate genes
Elenco autori:
V. Ricciardi, M. Crespan, G. Maddalena, D. Migliaro, L. Brancadoro, D. Maghradze, O. Failla, S.L. Toffolatti, G. De Lorenzis
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