Data di Pubblicazione:
2023
Citazione:
A marine-derived collagen-based skin substitute for skin regeneration: a preliminary study / L. Melotti, A. Perazzi, T. Martinello, I. Iacopetti, M. Sugni, M. Patruno. ((Intervento presentato al convegno TERMIS EU-Chapter tenutosi a Manchester nel 2023.
Abstract:
Skin wounds are a challenging problem in the medical field and an economical
burden. Skin substitutes (SS) are a promising alternative for skin wound healing since they mimic the
natural skin structure and support its regeneration. Here, we present preliminary results about the
application of an innovative marine-derived collagen-based SS in a secondary intention wound
healing model.
METHODS: The collagen used to produce the SS was obtained from sea urchin (Paracentrotus lividus)
food industry wastes, extracted in its fully native fibrillar and GAG-decorated form [1]. Two wounds
(4x4 cm) were surgically induced on the back of three sheep: one of them was treated with the SS
and one left untreated (placebo). Clinical evaluations for wound contraction and re-epithelialization
were performed every week. At 7, 14, 21 and 42 days, biopsies were collected with a 6 mm2 punch in
order to perform histopathological (H&E) and molecular (qPCR) analyses. Histological analyses
allowed to provide a score to the maturation of granulation tissue (GT) and skin adnexa. Gene
expression analysis was performed for Collagen1, Collagen3, VEGF and hair-keratin (hKER).
RESULTS: Clinically, the SS led to a better re-epithelialization than the placebo while no differences in
wound contraction were observed. Treated wounds showed a faster histological resolution of GT at
21 days, with no exuberant deposition of scarring tissue at 42 days; this result was further
corroborated by the different expression levels of both collagen types at 14 and 21 days.
Concomitantly, the higher expression of VEGF (day 14) was detected. In untreated wounds, on the
contrary, GT appeared only at 14 days and did not mature properly leading to dermal fibrosis at 42
days. SS application led to the development of a larger amount and better developed skin adnexa
(present since day 14) than untreated wounds: treated wounds started to express hKER since day 14.
DISCUSSION & CONCLUSIONS: At 42 days, a faster re-epithelialization, a better development of skin
adnexa and a properly deposition of GT were observed in the SS-treated wounds while the untreated
ones showed a hypertrophic epidermis and fibrosis, indicators of a tissue still in the process of
healing. Interestingly, VEGF expression might have supported GT maturation. Therefore, our
preliminary findings suggest an overall better skin repair of the SS treatment than the placebo.
Future studies should compare these beneficial effects with a commercially available SS (Integra®).
REFERENCES: [1] Ferrario et al. Mar Environ Res. Jul;128:46-57, 2017
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
L. Melotti, A. Perazzi, T. Martinello, I. Iacopetti, M. Sugni, M. Patruno
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