Data di Pubblicazione:
2021
Citazione:
A first exon termination checkpoint preferentially suppresses extragenic transcription / L. Austenaa, V. Piccolo, M. Russo, E. Prosperini, S. Polletti, D. Polizzese, S. Ghisletti, I. Barozzi, G. Diaferia, G. Natoli. - In: NATURE STRUCTURAL & MOLECULAR BIOLOGY. - ISSN 1545-9993. - 28:4(2021), pp. 337-346. [10.1038/s41594-021-00572-y EA MAR 2021]
Abstract:
Interactions between the splicing machinery and RNA polymerase II increase protein-coding gene transcription. Similarly, exons and splicing signals of enhancer-generated long noncoding RNAs (elncRNAs) augment enhancer activity. However, elncRNAs are inefficiently spliced, suggesting that, compared with protein-coding genes, they contain qualitatively different exons with a limited ability to drive splicing. We show here that the inefficiently spliced first exons of elncRNAs as well as promoter-antisense long noncoding RNAs (pa-lncRNAs) in human and mouse cells trigger a transcription termination checkpoint that requires WDR82, an RNA polymerase II–binding protein, and its RNA-binding partner of previously unknown function, ZC3H4. We propose that the first exons of elncRNAs and pa-lncRNAs are an intrinsic component of a regulatory mechanism that, on the one hand, maximizes the activity of these cis-regulatory elements by recruiting the splicing machinery and, on the other, contains elements that suppress pervasive extragenic transcription.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
L. Austenaa, V. Piccolo, M. Russo, E. Prosperini, S. Polletti, D. Polizzese, S. Ghisletti, I. Barozzi, G. Diaferia, G. Natoli
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