Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli in a Dairy Herd: Distribution and Antimicrobial Resistance Profiles
Articolo
Data di Pubblicazione:
2024
Citazione:
Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli in a Dairy Herd: Distribution and Antimicrobial Resistance Profiles / M. Penati, L. Musa, L. Filippone Pavesi, A. Guaraglia, F. Ulloa, P. Moroni, R. Piccinini, M.F. Addis. - In: ANTIBIOTICS. - ISSN 2079-6382. - 13:3(2024 Mar 05), pp. 241.1-241.10. [10.3390/antibiotics13030241]
Abstract:
This study investigated the presence, distribution, and antimicrobial resistance profiles
of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in a dairy herd located in
Northern Italy. The feces of clinically healthy calves, their mothers, and the cows treated for mastitis,
as well as water, environmental samples, and waste milk were collected and subjected to bacterio-
logical culture on CHROMagarTM ESBL plates. A questionnaire was administered to identify risk
factors. The isolates were identified as E. coli by MALDI-TOF MS and subjected to the double-disk
synergy test (DDST) and minimal inhibitory concentration (MIC) assay. As a result, ESBL E. coli
was isolated from the feces of 28 of 37 (75.67%) calves, the feces of 2 of 3 (66.67%) treated cows, 8 of
14 (57.15%) environmental samples, and waste milk. All ESBL isolates showed multiple resistances
and were categorized as multidrug-resistant (MDR). Several risk factors for ESBL E. coli selection and
diffusion were identified, including lack of routine cleaning of calf feeding and housing equipment,
administration of waste milk to male calves, and blanket dry cow therapy. In conclusion, this study
highlighted the presence of MDR, ESBL E. coli in the feces of most dairy calves, and their association
with different sample sources. Accordingly, adding to the prudent use of antibiotics, the adoption of
adequate farm hygiene and biosecurity measures might also help prevent the spread and transmission
of ESBL E. coli within the herd.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
antimicrobial resistance; biosecurity; dairy calves; ESBL E. coli; multidrug-resistant; waste milk
Elenco autori:
M. Penati, L. Musa, L. Filippone Pavesi, A. Guaraglia, F. Ulloa, P. Moroni, R. Piccinini, M.F. Addis
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