Serum antibody fingerprinting of SARS-CoV-2 variants in infected and vaccinated subjects by label-free microarray biosensor
Articolo
Data di Pubblicazione:
2024
Citazione:
Serum antibody fingerprinting of SARS-CoV-2 variants in infected and vaccinated subjects by label-free microarray biosensor / T. Carzaniga, L. Casiraghi, G. Nava, G. Zanchetta, T. Inzani, M. Chiari, V. Bollati, S. Epis, C. Bandi, A. Lai, G. Zehender, T. Bellini, M. Buscaglia. - In: FRONTIERS IN IMMUNOLOGY. - ISSN 1664-3224. - 15:(2024 Feb), pp. 1323406.1-1323406.11. [10.3389/fimmu.2024.1323406]
Abstract:
Both viral infection and vaccination affect the antibody repertoire of a person.
Here, we demonstrate that the analysis of serum antibodies generates
information not only on the virus type that caused the infection but also on
the specific virus variant. We developed a rapid multiplex assay providing a
fingerprint of serum antibodies against five different SARS-CoV-2 variants
based on a microarray of virus antigens immobilized on the surface of a label-
free reflectometric biosensor. We analyzed serum from the plasma of
convalescent subjects and vaccinated volunteers and extracted individual
antibody profiles of both total immunoglobulin Ig and IgA fractions. We found
that Ig level profiles were strongly correlated with the specific variant of infection
or vaccination and that vaccinated subjects displayed a larger quantity of total Ig
and a lower fraction of IgA relative to the population of convalescent
unvaccinated subjects.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
label-free biosensor, reflective phantom interface, antibody repertoire, rapid detection, wash-free assay, serological assay, immunoglobulins, IgA
Elenco autori:
T. Carzaniga, L. Casiraghi, G. Nava, G. Zanchetta, T. Inzani, M. Chiari, V. Bollati, S. Epis, C. Bandi, A. Lai, G. Zehender, T. Bellini, M. Buscaglia
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