Anthocyanins confer cardioprotection against Doxorubicin by modulating the AMPK-SIRT1-p53 pathway
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Data di Pubblicazione:
2022
Citazione:
Anthocyanins confer cardioprotection against Doxorubicin by modulating the AMPK-SIRT1-p53 pathway / D. Zorzan, F. Cappellini, A. Marinelli, M. Toccaceli, A. Bucchi, C. Tonelli, K. Petroni. ((Intervento presentato al 16. convegno Congress of the Italian Federation of Life Sciences (FISV) tenutosi a Portici nel 2022.
Abstract:
Doxorubicin (Doxo) is one of the most effective chemotherapeutic drugs, but its clinical use is severely limited by its side effects, leading to cardiomyopathy and heart failure. An anthocyanin (ACN)-rich diet from purple corn (RED diet), which mainly contains cyanidin 3-glucoside (C3G) and its acetylated derivatives, was proved to be effective in reducing Doxo-induced cardiotoxicity in mice. Aiming at unveiling the molecular mechanisms involved in ACN protection, we decided to consider the AMPK-SIRT1-p53 pathway that recently gained interest for its cardioprotective role. HL-1 murine cardiomyocytes were treated with Doxo in presence or absence of purple corn extract (RED) and activation level of AMPK, SIRT1, p53 and its apoptosis-related target genes were evaluated. Our results showed that RED decreased Doxo-induced AMPK activation and increased p53 acetylation, which resulted in decreased levels of cleaved-Caspase 3, Puma and p21 transcripts and apoptosis. The RED-induced p53 acetylation and its cardioprotective role is currently under validation in mouse primary cardiomyocytes. In conclusion, RED may prevent cardiomyocytes apoptosis through AMPK and SIRT1 modulation.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
D. Zorzan, F. Cappellini, A. Marinelli, M. Toccaceli, A. Bucchi, C. Tonelli, K. Petroni
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