A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscle
Articolo
Data di Pubblicazione:
2008
Citazione:
A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscle / L. Berghella, L. De Angelis, T. De Buysscher, A. Mortazavi, S. Biressi, S.V. Forcales, D. Sirabella, G. Cossu, B.J. Wold. - In: GENES & DEVELOPMENT. - ISSN 0890-9369. - 22:15(2008), pp. 2125-2138. [10.1101/gad.468508]
Abstract:
Myogenin is the dominant transcriptional regulator of embryonic and fetal muscle differentiation and during
maturation is profoundly down-regulated. We show that a highly conserved 17-bp DNA cis-acting sequence
element located upstream of the myogenin promoter (myogHCE) is essential for postnatal repression of
myogenin in transgenic animals. We present multiple lines of evidence supporting the idea that repression is
mediated by the Y-box protein MSY-3. Electroporation in vivo shows that myogHCE and MSY-3 are required
for postnatal repression. We further show that, in the C2C12 cell culture system, ectopic MSY-3 can repress
differentiation, while reduced MSY-3 promotes premature differentiation. MSY-3 binds myogHCE
simultaneously with the homeodomain protein Pbx in postnatal innervated muscle. We therefore propose a
model in which the myogHCE motif operates as a switch by specifying opposing functions; one that was
shown previously is regulated by MyoD and Pbx and it specifies a chromatin opening, gene-activating function
at the time myoblasts begin to differentiate; the other includes MYS-3 and Pbx, and it specifies a repression
function that operates during and after postnatal muscle maturation in vivo and in myoblasts before they
begin to differentiate.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
CsdA ; MSY-3 ; myogenin ; Y-box ; innervation ; muscle maturation
Elenco autori:
L. Berghella, L. De Angelis, T. De Buysscher, A. Mortazavi, S. Biressi, S.V. Forcales, D. Sirabella, G. Cossu, B.J. Wold
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