Changes in the proteomic and metabolomic profiles of Beta vulgaris root tips in response to iron deficiency and resupply
Articolo
Data di Pubblicazione:
2010
Citazione:
Changes in the proteomic and metabolomic profiles of Beta vulgaris root tips in response to iron deficiency and resupply / R. Rellan-Alvarez, S. Andaluz, J. Rodriguez-Celma, G. Wohlgemuth, G. Zocchi, A. Alvarez-Fernandez, O. Fiehn, A. Lopez-Millan, J. Abadia. - In: BMC PLANT BIOLOGY. - ISSN 1471-2229. - 10:120(2010), pp. 120.1-120.15.
Abstract:
Background: Plants grown under iron deficiency show different morphological, biochemical and physiological
changes. These changes include, among others, the elicitation of different strategies to improve the acquisition of Fe
from the rhizosphere, the adjustment of Fe homeostasis processes and a reorganization of carbohydrate metabolism.
The application of modern techniques that allow the simultaneous and untargeted analysis of multiple proteins and
metabolites can provide insight into multiple processes taking place in plants under Fe deficiency. The objective of this
study was to characterize the changes induced in the root tip proteome and metabolome of sugar beet plants in
response to Fe deficiency and resupply.
Results: Root tip extract proteome maps were obtained by 2-D isoelectric focusing polyacrylamide gel
electrophoresis, and approximately 140 spots were detected. Iron deficiency resulted in changes in the relative
amounts of 61 polypeptides, and 22 of them were identified by mass spectrometry (MS). Metabolites in root tip
extracts were analyzed by gas chromatography-MS, and more than 300 metabolites were resolved. Out of 77 identified
metabolites, 26 changed significantly with Fe deficiency. Iron deficiency induced increases in the relative amounts of
proteins and metabolites associated to glycolysis, tri-carboxylic acid cycle and anaerobic respiration, confirming
previous studies. Furthermore, a protein not present in Fe-sufficient roots, dimethyl-8-ribityllumazine (DMRL) synthase,
was present in high amounts in root tips from Fe-deficient sugar beet plants and gene transcript levels were higher in
Fe-deficient root tips. Also, a marked increase in the relative amounts of the raffinose family of oligosaccharides (RFOs)
was observed in Fe-deficient plants, and a further increase in these compounds occurred upon short term Fe resupply.
Conclusions: The increases in DMRL synthase and in RFO sugars were the major changes induced by Fe deficiency
and resupply in root tips of sugar beet plants. Flavin synthesis could be involved in Fe uptake, whereas RFO sugars
could be involved in the alleviation of oxidative stress, C trafficking or cell signalling. Our data also confirm the increase
in proteins and metabolites related to carbohydrate metabolism and TCA cycle pathways.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
R. Rellan-Alvarez, S. Andaluz, J. Rodriguez-Celma, G. Wohlgemuth, G. Zocchi, A. Alvarez-Fernandez, O. Fiehn, A. Lopez-Millan, J. Abadia
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