Characterisation of Matrix-Bound Nanovesicles (MBVs) Isolated from Decellularised Bovine Pericardium: New Frontiers in Regenerative Medicine
Articolo
Data di Pubblicazione:
2024
Citazione:
Characterisation of Matrix-Bound Nanovesicles (MBVs) Isolated from Decellularised Bovine Pericardium: New Frontiers in Regenerative Medicine / D. Di Francesco, C. Di Varsavia, S. Casarella, E. Donetti, M. Manfredi, D. Mantovani, F. Boccafoschi. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 25:2(2024 Jan), pp. 740.1-740.17. [10.3390/ijms25020740]
Abstract:
Matrix-bound nanovesicles (MBVs) are a recently discovered type of extracellular vesicles
(EVs), and they are characterised by a strong adhesion to extracellular matrix structural proteins
(ECM) and ECM-derived biomaterials. MBVs contain a highly bioactive and tissue-specific cargo
that recapitulates the biological activity of the source ECM. The rich content of MBVs has shown to
be capable of potent cell signalling and of modulating the immune system, thus the raising interest
for their application in regenerative medicine. Given the tissue-specificity and the youthfulness
of research on MBVs, until now they have only been isolated from a few ECM sources. Therefore,
the objective of this research was to isolate and identify the presence of MBVs in decellularised
bovine pericardium ECM and to characterise their protein content, which is expected to play a
major role in their biological potential. The results showed that nanovesicles, corresponding to the
definition of recently described MBVs, could be isolated from decellularised bovine pericardium
ECM. Moreover, these MBVs were composed of numerous proteins and cytokines, thus preserving a
highly potential biological effect. Overall, this research shows that bovine pericardium MBVs show a
rich and tissue-specific biological potential.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
matrix-bound nanovesicles (MBVs); extracellular vesicles; decellularised extracellular matrix; extracellular matrix biomaterials
Elenco autori:
D. Di Francesco, C. Di Varsavia, S. Casarella, E. Donetti, M. Manfredi, D. Mantovani, F. Boccafoschi
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