Aedes albopictus microbiota: Differences between wild and mass-reared immatures do not suggest negative impacts from a diet based on black soldier fly larvae and fish food
Articolo
Data di Pubblicazione:
2023
Citazione:
Aedes albopictus microbiota: Differences between wild and mass-reared immatures do not suggest negative impacts from a diet based on black soldier fly larvae and fish food / C. Polidori, A. Ferrari, L. Borruso, P. Mattarelli, M.L. Dindo, M. Modesto, M. Carrieri, A. Puggioli, F. Ronchetti, R. Bellini. - In: PLOS ONE. - ISSN 1932-6203. - 18:9(2023), pp. e0292043.1-e0292043.15. [10.1371/journal.pone.0292043]
Abstract:
The “Sterile Insect Technique” (SIT), a promising method to control Aedes albopictus, the
Asian tiger mosquito, is gaining increasing interest. Recently, the role of microbiota in mos-
quito fitness received attention, but the link between microbiota and larval diet in mass rear-
ing programs for SIT remains largely unexplored. We characterized the microbiota of four
larval instars, pupae and eggs of non-wild (NW) lab-reared Ae. albopictus fed with a diet
based on Black soldier fly (Hermetia illucens) larvae powder and fish food KOI pellets. We
compared it with wild (W) field-collected individuals and the bacterial community occurring
in rearing water-diet (DIET). A total of 18 bacterial classes with > 0.10% abundance were
found overall in the samples, with seven classes being especially abundant. Overall, the
microbiota profile significantly differed among NW, W and DIET. Verrucomicrobiae were sig-
nificantly more abundant in W and DIET, Bacteroidia were more abundant in NW and DIET,
and Gammaproteobacteria were only more abundant in W than in DIET. W-eggs microbiota
differed from all the other groups. Large differences also appeared at the bacterial genus-
level, with the abundance of 14 genera differing among groups. Three ASVs of Acinetobac-
ter, known to have positive effects on tiger mosquitoes, were more abundant in NW than in
W, while Serratia, known to have negative or neutral effects on another Aedes species, was
less abundant in NW than in W. The bacterial community of W-eggs was the richest in spe-
cies, while dominance and diversity did not differ among groups. Our data show that the diet
based on Black soldier fly powder and fish food KOI influences the microbiota of NW tiger
mosquito immature stages, but not in a way that may suggest a negative impact on their
quality in SIT programs.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
C. Polidori, A. Ferrari, L. Borruso, P. Mattarelli, M.L. Dindo, M. Modesto, M. Carrieri, A. Puggioli, F. Ronchetti, R. Bellini
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