The Neurotoxic Effect of Environmental Temperature Variation in Adult Zebrafish (Danio rerio)
Articolo
Data di Pubblicazione:
2023
Citazione:
The Neurotoxic Effect of Environmental Temperature Variation in Adult Zebrafish (Danio rerio) / E. Maffioli, S. Nonnis, F. Grassi Scalvini, A. Negri, G. Tedeschi, M. Toni. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 24:21(2023), pp. 15735.1-15735.23. [10.3390/ijms242115735]
Abstract:
Neurotoxicity consists of the altered functionality of the nervous system caused by ex-
posure to chemical agents or altered chemical–physical parameters. The neurotoxic effect can be
evaluated from the molecular to the behavioural level. The zebrafish Danio rerio is a model organism
used in many research fields, including ecotoxicology and neurotoxicology. Recent studies by our
research group have demonstrated that the exposure of adult zebrafish to low (18 ◦C) or high (34 ◦C)
temperatures alters their brain proteome and fish behaviour compared to control (26 ◦C). These
results showed that thermal variation alters the functionality of the nervous system, suggesting a
temperature-induced neurotoxic effect. To demonstrate that temperature variation can be counted
among the factors that generate neurotoxicity, eight different protein datasets, previously published
by our research group, were subjected to new analyses using an integrated proteomic approach by
means of the Ingenuity Pathway Analysis (IPA) software (Release December 2022). The datasets con-
sist of brain proteome analyses of wild type adult zebrafish kept at three different temperatures (18 ◦C,
26 ◦C, and 34 ◦C) for 4 days (acute) or 21 days (chronic treatment), and of BDNF+/− and BDNF−/−
zebrafish kept at 26 ◦C or 34 ◦C for 21 days. The results (a) demonstrate that thermal alterations
generate an effect that can be defined as neurotoxic (p value ≤ 0.05, activation Z score ≤ −2 or ≥2),
(b) identify 16 proteins that can be used as hallmarks of the neurotoxic processes common to all the
treatments applied and (c) provide three protein panels (p value ≤ 0.05) related to 18 ◦C, 34 ◦C, and
BDNF depletion that can be linked to anxiety-like or boldness behaviour upon these treatments.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
neurotoxicity; temperature; zebrafish; BDNF; proteomic; behaviour
Elenco autori:
E. Maffioli, S. Nonnis, F. Grassi Scalvini, A. Negri, G. Tedeschi, M. Toni
Link alla scheda completa: