Data di Pubblicazione:
2009
Citazione:
Synergistic interactions of antioxidant nutrients in a biological model system / K.J. Yeum, G. Beretta, N.I. Krinsky, R.M. Russell, G. Aldini. - In: NUTRITION. - ISSN 0899-9007. - 25:7-8(2009), pp. 839-846.
Abstract:
Reconstituted human serum consisting of delipidized human serum (DHS) combined with phosphatidylcholine liposomes (PCL) was used to determine antioxidant activities of physiologic concentrations of antioxidant nutrients. Radicals were initiated with 2,2′-azobis(4-methoxy-2,4-dimethylvaleronitrile) (2 mmol/L), and oxidation was monitored by 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid. Fat-soluble antioxidant nutrients were incorporated into the PCL prepared by sonication and suspended in DHS to avoid any interference by the endogenous fat-soluble antioxidants. Water-soluble antioxidants were added directly into the DHS. The oxidation kinetics were monitored every 5 min up to 210 min using a microplate reader (excitation wavelength 500 nm, emission wavelength 520 nm).
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Antioxidant network; Synergistic interaction; Total antioxidant performance
Elenco autori:
K.J. Yeum, G. Beretta, N.I. Krinsky, R.M. Russell, G. Aldini
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