Immobilized biocatalysts for the production of nucleosides and nucleoside analogues by enzymatic transglycosylation reactions
Articolo
Data di Pubblicazione:
2004
Citazione:
Immobilized biocatalysts for the production of nucleosides and nucleoside analogues by enzymatic transglycosylation reactions / G. Zuffi, D. Ghisotti, I. Oliva, E. Capra, G. Frascotti, G. Tonon, G. Orsini. - In: BIOCATALYSIS AND BIOTRANSFORMATION. - ISSN 1024-2422. - 22:1(2004), pp. 25-33. [10.1080/10242420310001648551]
Abstract:
The recombinant enzymes uridine phosphorylase (UP) and purine nucleoside phosphorylase (PNP) were over-expressed in high-biomass bacterial fermentations and co-immobilized, without previous purification, on epoxy-activated solid supports by covalent linkages. These preparations are efficient biocatalysts of transglycosylation reactions and have been developed for producting natural and modified nucleosides of pharmaceutical interest in the field of antiviral and antitumoral agents. The new biocatalysts described in this work are suitable for both laboratory and industrial scale applications due to the maintainance of high catalytic efficiency, thermal and solvent stability, reusability and ease of operation in batch as well as in continuous reactions.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
nucleosides ; transglycosylation ; transformed Escherichia coli ; immobilized enzymes ; uridine phosphorylase (EC2.4.2.3) ; purine nucleoside phosphorylase (EC2.4.2.1)
Elenco autori:
G. Zuffi, D. Ghisotti, I. Oliva, E. Capra, G. Frascotti, G. Tonon, G. Orsini
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