Unravelling Novel SCN5A Mutations Linked to Brugada Syndrome: Functional, Structural, and Genetic Insights
Articolo
Data di Pubblicazione:
2023
Citazione:
Unravelling Novel SCN5A Mutations Linked to Brugada Syndrome: Functional, Structural, and Genetic Insights / A. Frosio, E. Micaglio, I. Polsinelli, S. Calamaio, D. Melgari, R. Prevostini, A. Ghiroldi, A. Binda, P. Carrera, M. Villa, F. Mastrocinque, S. Presi, R. Salerno, A. Boccellino, L. Anastasia, G. Ciconte, S. Ricagno, C. Pappone, I. Rivolta. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 24:20(2023), pp. 15089.1-15089.17. [10.3390/ijms242015089]
Abstract:
Brugada Syndrome (BrS) is a rare inherited cardiac arrhythmia causing potentially fatal
ventricular tachycardia or fibrillation, mainly occurring during rest or sleep in young individuals
without heart structural issues. It increases the risk of sudden cardiac death, and its characteristic
feature is an abnormal ST segment elevation on the ECG. While BrS has diverse genetic origins, a
subset of cases can be conducted to mutations in the SCN5A gene, which encodes for the Nav1.5
sodium channel. Our study focused on three novel SCN5A mutations (p.A344S, p.N347K, and
p.D349N) found in unrelated BrS families. Using patch clamp experiments, we found that these
mutations disrupted sodium currents: p.A344S reduced current density, while p.N347K and p.D349N
completely abolished it, leading to altered voltage dependence and inactivation kinetics when co-
expressed with normal channels. We also explored the effects of mexiletine treatment, which can
modulate ion channel function. Interestingly, the p.N347K and p.D349N mutations responded well
to the treatment, rescuing the current density, while p.A344S showed a limited response. Structural
analysis revealed these mutations were positioned in key regions of the channel, impacting its stability
and function. This research deepens our understanding of BrS by uncovering the complex relationship
between genetic mutations, ion channel behavior, and potential therapeutic interventions.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Brugada syndrome; sudden cardiac death; arrhythmias; sodium channel; Nav1.5; SCN5A; automated patch clamp; mexiletine
Elenco autori:
A. Frosio, E. Micaglio, I. Polsinelli, S. Calamaio, D. Melgari, R. Prevostini, A. Ghiroldi, A. Binda, P. Carrera, M. Villa, F. Mastrocinque, S. Presi, R. Salerno, A. Boccellino, L. Anastasia, G. Ciconte, S. Ricagno, C. Pappone, I. Rivolta
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